Międzyresortowy Instytut Techniki Radiacyjnej Prof. dr hab. Halina Abramczyk Dr hab. inż. Beata Brożek-Płuska Dr inż. Jakub Surmacki Mgr inż. Monika Kopeć.

Slides:



Advertisements
Similar presentations
Scanning near-field optical microscopy (SNOM) for magneto-optics Paolo Vavassori INFM - National Research Center on nanoStructures and Biosystems at Surfaces.
Advertisements

Measuring Nanostructures. How do we see nanostructures? A light microscope? Helpful, but cannot resolve below 1000 nm An electron microscope? Has a long.
Bragg’s Law nl=2dsinΘ Just needs some satisfaction!! d Θ l
Contact: Annika Enejder, Technical BioImaging TIF045 5p 24:th October-9:th December To see is to understand...
Fire Protection Laboratory Methods Day
Scanning Probe Microscopy
Atomic Force Microscopy Andrew Rouff and Kyle Chau.
SCANNING PROBE MICROSCOPY By AJHARANI HANSDAH SR NO
Fluorophores bound to the specimen surface and those in the surrounding medium exist in an equilibrium state. When these molecules are excited and detected.
Lecture 10. AFM.
AFM-Raman and Tip Enhanced Raman studies of modern nanostructures Pavel Dorozhkin, Alexey Shchekin, Victor Bykov NT-MDT Co., Build. 167, Zelenograd Moscow,
Apertureless Scanning Near-field Optical Microscopy: a comparison between homodyne and heterodyne approaches Journal Club Presentation – March 26 th, 2007.
Development of Local and Scanning Probe Techniques Heinrich Hoerber NanoBioPhysics University Bristol.
STED: Nanoscale 3D Optical Imaging Digvijay Raorane & Arun Majumdar Department of Mechanical Engineering Department of Materials Science University of.
Activities during UK-Japan Young Scientist Workshop Dr Riz Khan Room 31DJ02, x6062, Advanced Technology Institute University.
Pump-Probe Spectroscopy Chelsey Dorow Physics 211a.
Institute of Optics, University of Rochester1 Carbon Nanotubes: theory and applications Yijing Fu 1, Qing Yu 2 1 Institute of Optics, University of Rochester.
Atomic Force Microscop (AFM) 3 History and Definitions in Scanning Probe Microscopy (SPM) History Scanning Tunneling Microscope (STM) Developed.
Copyright © 2005 SRI International Scanning Probe Microscopy “Seeing” at the nanoscale.
Scanning Probe Microscopy (SPM) Real-Space Surface Microscopic Methods.
Common types of spectroscopy
Ultrafast Experiments Hangwen Guo Solid State II Department of Physics & Astronomy, The University of Tennessee.
‘Wet’ Chemical Techniques One technique to analyze the chemistry of a mineral is to dissolve it –Water, Strong acids/bases, hydrofluoric acid, oxidants,
Nano-technology and Nano-electronics
Three-dimensional Silicon composite nanostructures, taken with a scanning electron microscope.
Demolding ENGR Pre Lab.
Attosecond Light and Science at the Time-scale of the Electron –
April 2004 Developing Optical Techniques for Analysis of Materials at the Nanoscale Alexei Sokolov.
Defect Review in the Photonics Revolution Aaron Lewis Nanonics Imaging Ltd. The Manhat Technology Park Malcha, Jerusalem ISRAEL Tel:
MRI: Development of a New Paradigm for Apertureless Near-field Scanning Optical Microscope Gang-yu Liu, University of California, Davis, DMR We.
Tao Yuan, Jingzhou Xu, and Xicheng Zhang Rensselaer Polytechnic Institute, Troy, New York Scanning THz Emission Microscope Abstract A THz image system.
J.R.Krenn – Nanotechnology – CERN 2003 – Part 3 page 1 NANOTECHNOLOGY Part 3. Optics Micro-optics Near-Field Optics Scanning Near-Field Optical Microscopy.
Reminders for this week Homework #4 Due Wednesday (5/20) Lithography Lab Due Thursday (5/21) Quiz #3 on Thursday (5/21) – In Classroom –Covers Lithography,
Scanning Probe Microscopy Colin Folta Matt Hense ME381R 11/30/04.
Engr College of Engineering Engineering Education Innovation Center Engr 1182 Nano Pre-Lab Demolding Rev: 20XXMMDD, InitialsPresentation Short.
Ferroelectric Nanolithography Extended to Flexible Substrates Dawn A. Bonnell, University of Pennsylvania, DMR Recent advances in materials synthesis.
Characterization of Nanomaterials…
Optics Focus Confocal theory.
Nanolithography Using Bow-tie Nanoantennas Rouin Farshchi EE235 4/18/07 Sundaramurthy et. al., Nano Letters, (2006)
Using Technology to Study Cellular and Molecular Biology.
Electron Microscope. How do they work Instead of using light they fire a beam of electrons (which have a wavelength less than 1nm compared to light which.
3.052 Nanomechanics of Materials and Biomaterials Prof. Christine Ortiz DMSE, RM Phone : (617) WWW :
Near Field Scanning Optical Microscopy (NSOM, SNOM, NFOM) Stephanie Pruzinsky Group Meeting, June 6, 2002.
Raman spectroscopy.
Introduction of Nanoplasmonics 2011 Spring Semester.
Developing Confocal Raman-AFM and Fluorescence-AFM Imaging Techniques to Visualize Drug-Cell Interactions with Further Implications in Cellular Pathology.
METHODOLOGY Nanotechnology Prof. Dr. Abdul Majid Department of Physics
RAMAN EFFECT.
Coherent spectroscopy Kevin Crampton Alex Fast Alba Alfonso-Garcia Christopher Syme Ara Apkarian Eric O. Potma in the single molecule limit.
SHINE: S eattle’s H ub for I ndustry-driven N anotechnology E ducation North Seattle College Nanotechnology Characterization.
Non-linear photochromism in the near-field of a nanoplasmonic array
SEMINAR 1. Title : Nanoscale Optical Spectroscopy
3.3 Other types of microscopy
Scanning Probe Microscopy
NANO 230 Micro/NanoFabrication
MultiView 400™ Product Presentation Nanonics MultiView 400™
Near-Field Optical Microscopy
Surface-Sensitive Raman Spectroscopy of Collagen I Fibrils
Nanocharacterization (III)
Scalar theory of diffraction
Scanning Near-Field Fluorescence Resonance Energy Transfer Microscopy
Types of Microscopy Type Probe Technique Best Resolution Penetration
Scanning Probe Microscopy
FLUORESCENCE MICROSCOPY
Atomic Force Microscope
Planck’s law: E=hn =hc/l
Magnetic force resonance microscopy
Alan Van Orden, Department of Chemistry, Colorado State University
Fig. 1 Near-field ultrafast and broadband pump-probe of EP in WSe2.
Fig. 1 Plasmonic pumping experiment and photoinduced near-field optical response in Hg0.81Cd0.19Te. Plasmonic pumping experiment and photoinduced near-field.
Presentation transcript:

Międzyresortowy Instytut Techniki Radiacyjnej Prof. dr hab. Halina Abramczyk Dr hab. inż. Beata Brożek-Płuska Dr inż. Jakub Surmacki Mgr inż. Monika Kopeć Halina Abramczyk Lodz University of Technology, Laboratory of Laser Molecular Spectroscopy, Lodz, Poland

: Laboratory of Laser Molecular Spectroscopy : Laboratory of Raman Imaging Laboratory of Femtosecond Spectroscopy Laboratory of Raman Spectroscopy

Biomedical applications High spatial resolution (far below the diffraction limit, TERS) RAMAN IMAGING High temporal resolution (FEMTOSECOND PUMP-PROBE SPECTROSCOPY) Strong signal enhancement enabling monitoring the genetic and immunological responses in biological systems (SERS COMBINED WITH NANOPARTICLES) Specificity of interactions (BIOCONJUGATES)

Biomedical applications High spacial resolution (far below the diffraction limit, TERS) RAMAN IMAGING

CONFOCAL RAMAN MICROSCOPY R>> wide-field microscopy

Scanning near-field microscopy (SNOM) Near-field imaging occurs when a sub-micron optical probe is positioned at a very short distance from the sample and light is transmitted through a small aperture at the tip of this probe. The near-field is defined as the region above a surface with dimensions less than a single wavelength of the light incident on the surface. Within the near-field region light is not diffraction limited and nanometer spatial resolution is possible. This phenomenon enables non-diffraction limited imaging of a sample that is simply not possible with conventional optical imaging techniques. d<< The next step to material analysis on a smaller scale has been the combination of Raman spectroscopic analysis with near field optics and an Atomic force microscope (AFM). Such systems allow tip enhanced Raman scattering to be explored, making true NanoRaman achievable, with spatial resolution <100nm. spatial resolution <100nm.

Tip-enhanced Raman spectroscopy (TERS) (cantilever based SNOM) In a typical TERS experiment a Au- or Ag-coated AFM tip is used as a nanostructure to produce Raman signal enhancement on a sample surface once the excitation laser is focused on the apex of the tip with the tip brought into close proximity with the surface. The tip radius, which defines the lateral resolution of an AFM measurement, is typically in the range of nm. In the TERS experiment the lateral resolution depends on the size of the hot-spot therefore one can expect resolution in the range of nm for Raman spectroscopy and imaging measurements. The TERS tip-apex must be illuminated with the excitation laser from either above, below, or the side.

Biomedical applications High spatial resolution (far below the diffraction limit, TERS) RAMAN IMAGING High temporal resolution (FEMTOSECOND PUMP- PROBE SPECTROSCOPY)

Ultrafast nonlinear spectroscopy pump-probe femtosecond transient absorption Recently developed techniques of ultrafast nonlinear vibrational spectroscopy allow a much more effective attack on this problem.

Biomedical applications High spacial resolution (far below the diffraction limit, TERS) RAMAN IMAGING High temporal resolution (FEMTOSECOND PUMP-PROBE SPECTROSCOPY) Strong signal enhancement enabling monitoring the genetic and immunological responses in biological systems (SERS COMBINED WITH NANOPARTICLES) Specificity of interactions (BIOCONJUGATES)

H. Abramczyk, B. Brozek-Pluska, Chem. Rev. 113 (2013) IF = 41,298

16 października

H. Abramczyk, B. Brozek-Pluska, J. Surmacki, J. Jablonska-Gajewicz, R. Kordek, PBMB 108 (2012)

16 października

16 października

16 października

2014 H. Abramczyk, B. Brozek-Pluska, J. Surmacki, J. Musial, R. Kordek, Analyst (2014) DOI: /c4an00966e

J. Surmacki, B. Brozek-Pluska, J. Musial, R. Kordek, H. Abramczyk, Analyst (2015) DOI: /C4AN01876A 2015 ANALYST cover