Supplementary Fig. 1 Transmission electron micrographs of strains a CCR04 and b CCR80 grown on nutrient agar for 24 h. a b
Strain CCR80 (EU034656) Pseudomonas corrugata ATCC (PSEATCC11) Strain CCR04 (EU034655) Pseudomonas brassicacearum CFBP (AF100322) Pseudomonas fluorescens ATCC T (AJ308308) Pseudomonas marginalis ATCC T (AJ308309) Pseudomonas mediterranea CFBP 5447 T (AF386080) Pseudomonas syringae ATCC T (AJ308316) Pseudomonas chlororaphis ATCC 9446 T (AJ308301) Pseudomonas cichorii ATCC T (AJ308302) Pseudomonas graminis DSM T (Y11150) Pseudomonas agarici ICMP 2656 T (AJ308298) Pseudomonas fulva NRIC 0180 T (AB046998) Pseudomonas putida DSM 291 T (Z76667) Pseudomonas mendocina ATCC T (AJ308310) Pseudomonas anguilliseptica NCMB 1949 T (AB021376) Pseudomonas oryzihabitans IAM 1568 T (AM262973) Pseudomonas nitroreducens DSM T (AM088474) Pseudomonas otitidis MCC10330 T (AY953147) Pseudomonas aeruginosa ATCC (AF094713) Supplementary Fig. 2 A phylogenetic neighbor-joining tree showing the relationship of strains CCR04 and CCR80 with other members of the genus Pseudomonas. Bootstrap values (> 50 %) based on 1,000 replicates are shown at the branch points. The scale bar represents 1 nucleotide substitution per 1,000 nucleotides of the 16S rRNA sequence. Strains CCR04 and CCR80 tested in this study are indicated in bold types; GenBank accession numbers are in parenthesis. T, type strain of the species.
Treatment Microbial activity (µg hydrolyzed FDA min -1 g -1 dry wt. soil) d c b a a UntreatedDH5αAC-1CCR04CCR Supplementary Fig. 3 Total microbial activities by Pseudomonas corrugata strains CCR04 and CCR80 compared with 10 mM MgSO 4 solution (untreated control), Escherichia coli DH5α (negative control) and Paenibacillus polymyxa AC-1 (positive control) 2 weeks after treatment on 4-week-old pepper (cv. ‘Nockwang’) plants. Different letters on columns (mean + SE, n = 6) indicate significant differences between treatments at P < 0.05.