Proteomic, Metabolomic and Lipidomic Research Capability at ICH and ION UCL Label Free Quantitative Proteomics “biomarker discovery”

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Proteomic, Metabolomic and Lipidomic Research Capability at ICH and ION UCL Label Free Quantitative Proteomics “biomarker discovery”

Label-free quantitative proteomics using qTOF MS Sample, Tissue, Plasma Yeast Enolase (100 fmol) lyse Individual proteins

lyse Thousands of proteins Trypsin digest all proteins to peptides Tens/ hundreds of thousands of peptides QTOF Sequence everything Sample Label-free quantitative proteomics using qTOF MS

Time Amount of peptide (larger the response, more protein present)

Proteomic, Metabolomic and Lipidomic Research Capability at ICH and ION UCL

Proteomic, Metabolomic and Lipidomic Research Capability at ICH and ION UCL

Proteomic, Metabolomic and Lipidomic Research Capability at ICH and ION UCL

Proteomic, Metabolomic and Lipidomic Research Capability at ICH and ION UCL

Proteomic, Metabolomic and Lipidomic Research Capability at ICH and ION UCL

UCL Institute of Child Health & Great Ormond Street Hospital for Sick Children UCL

Proteomic, Metabolomic and Lipidomic Research Capability at ICH and ION UCL

Proteomic, Metabolomic and Lipidomic Research Capability at ICH and ION UCL

Proteomic, Metabolomic and Lipidomic Research Capability at ICH and ION UCL

Proteomic, Metabolomic and Lipidomic Research Capability at ICH and ION UCL

Acknowledgements UCL

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