Isotype control Supplementary Figure S1. Strategy for gating CD11b+CD14-CD33+CD40+ myeloid cells. Singlets were gated from live cells shown in forward.

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Supplementary Figures

Presentation transcript:

Isotype control Supplementary Figure S1. Strategy for gating CD11b+CD14-CD33+CD40+ myeloid cells. Singlets were gated from live cells shown in forward scatter height against forward scatter area. CD11b+ cells were first gated from the singlet gate. Next, CD14- cells were gated from the CD11b+ population and lastly CD33+ cells were gated from the CD11b+CD14- population. CD14+ monocytes were gated from the singlet gate. Gates for CD40+ cells were set after cells stained with isotype control antibodies.

Supplementary Figure S2. Strategy for NK cell gating Supplementary Figure S2. Strategy for NK cell gating. First live cells were gated. Subsequently, lymphocytes were gated from the live population and singlets were gated from the lymphocyte gate. NK cells, defined as CD3-CD56/CD16+ cells were gated from the singlet gate.

Supplementary Figure S3 Supplementary Figure S3. Strategy for gating naïve T cells, memory T cells and T regulatory cells. Lymphocytes were gated according to size and granularity. From the lymphocyte population live cells were gated. Subsequently, singlets were gated from this population. Upper panel: From the singlet gate, first CD3+ cells and then CD8+ cells were gated. Naïve T cells and memory T cells were gated from the CD8+ population and were defined as CD45RA+CCR7+ and CD45RA- respectively. Tregs (lower panel) were gated from singles by first gating the CD3+CD4+ cells. These cells were transferred to a plot for CD127 and FoxP3 to gate the FoxP3+CD127- T regulatory cell population.