Plasmolysis.

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Presentation transcript:

Plasmolysis

Some definitions Osmosis: Passage of water from a region of low concentration of solute to a region of high concentration, across a semipermeable membrane How is Osmosis different from Diffusion? Isotonic: Same solute concentration Hypotonic: Low solute concentration Hypertonic: High solute concentration Semipermeable membrane

Osmosis in blood cells

Osmosis in plant cells - Plasmolysis

Experiment To observe plasmolysis in Rhoeo discolor

Protocol Prepare sucrose dilutions from the 1M stock provided. Peel off a thin layer of the leaf from the purple side ( purple layer only) Put into the sucrose solution and wait for 20 minutes. Place the layer on the microscope slide, place a coverslip and observe the cells under microscope. Determine the number of plasmolysed cells in different concentrations of sucrose and estimate the isotonic concentration.

Sucrose concentration (M) 1 M sucrose (ml) H2O (ml) Number of plasmolysed cells Total number of cells % Plasmolysed 0.2 0.3 0.35 0.4 0.8 1.0