Carabao grass is a vigorous, creeping perennial grass with long stolons (branch) and rooting at nodes (Alberto and Sigua, 2013). Moreover, this grass is located around the Philippines. The medicinal use of this plant is its root and leaves. It is capable to inhibit staphylococcus that can cause skin infection ( 40.html, 2014).

With that, staphylococcal enteritis as an inflammatory disease caused by eating or drinking substances contaminated with staphylococcus enterotoxin.

Staphylococcal enterotoxin B (SEB) is an exotoxin produced by Staphylococcus aureus. It is one of the toxins responsible for staphylococcal food poisoning in humans and has been produced by some countries as a biological weapon ( aphylococcal_enterotoxin_b.pdf, 2004)

The toxin is not a bacterium that settles in the small intestine and cause inflammation and swelling. In this instance, it causes abdominal pain, cramping, dehydration, diarrhea and fever.

Thus, this study primarily aims to make an alternative solution using carabao grass leaves versus roots extracts as comparative samples to combat staphylococcus enterotoxin B that may attack to human immune system transmit by means of inhalation and ingestion. Through this endeavor we can be able to harness ways to fight the said

The research aims to answer the inquiry about the effectiveness of the comparative analysis between carbon grass leaf against its root extracts to inhibit staphylococcus enterotoxins samples. In other words, this general problem quest to look into the effectiveness of the carabao grass extracts against staphylococcus more particularly the staphylococcus enterotoxins.

Specifically the following are the inquiries for this endeavor: 1. At what level of effectiveness of carbon grass as to leaves versus roots extracts applied agents against staphylococcus enterotoxins in terms of it mixture with water (distilled) and methanol solutions in the following volumes in every 10 minutes mortality rate for inhibition: a. 5 ml; b. 10 ml; c. 15 ml; and d. 20 ml?

2. Is there a significant difference in applying carabao grass leaves extract with water and methanol solution (combination) against the roots extract with water and methanol solution (combination) to reduce the zone of inhibition of the staphylococcus enterotoxin?

3. Is there a significant relationship between the 10 minutes rate of mortality of staphylococcus enterotoxins to the designated volumes?

At 0.05 level of significance problem 2 and 3 were hypothesized as follows:  H o1 : There is no significant difference between the carabao grass leaves and roots separated extracts with water and methanol solution.  H o2 : There is no significant relationship between 10 minutes rate of mortality of staphylococcus enterotoxins to the designated volumes.

The following are the materials used for this study: 500 g carabao grass leaves 500 g carabao grass roots 1 pc. green grass extractor juicer 100 ml methanol solution 600 ml water (distilled) solution staphylococcus enterotoxin samples 2 pcs. petri dishes 1 pc. Microscope

3 pcs. beaker (500 ml) 1 pc. stirring rods 1 pc. graduated cylinder (100 ml) 1 pc. empty (clean) spray bottle 1 pc. Sharp knife 1 pc. Chopping board 1 pc. Bucket with water 1 pc. Whiteboard marker

The first step is the collection of carabao grass with roots. After wash the grass into the water until it will be cleaned with no soil and any dirt attached. Then, let the leaves and roots be separated using knife and chopping board. This time, extract the leaves using the green grass extractor juicer and use the beaker in filing the juice of the carabao grass leaves

After having the carabao grass leaf extract in the beaker and so with the root extract. In the preparation of the combined solution, set up the methanol and water (purified) solutions. Measure 40 ml methanol in a graduated cylinder and place it in the beaker. After which, add 40 ml purified water in the beaker with 40 ml methanol solution (not sudden pouring) using the stirring rod as it combines.

Now, the combined solution of water (purified) and methanol in the beaker will be placed to the filled leaf extract (20 ml- measured using graduated cylinder) in beaker using the stirring rod. After 5 minutes of combining the solution. Mark A (use the marker) for the beaker with the combined solutions of water, methanol and leaf extract. Then fill it now to the clean empty spray bottle. Same method will be done to the root extract. Mark B to the combined solutions of root extract.

Treat now the prepared agent (spray bottle) to the staphylococcus enterotoxins sepated sample with equal number of microorganism… Then, record the data.

VolumeT1T2T3T4T5 5 ml 10 ml 15 ml 20 ml Note: The allocated time after spraying is 10 minutes. Record only Yes or No if there is an inhibition of the staphylococcus enterotoxins.

Green Grass extraction juicer – 500 Php Staphylococcus enterotoxins samples Php Fare Php (Surigao City vice versa to Butuan City) Lab Fee Php 1260 Php

 Alberto, Annie Melinda P. and Sigua, Gilbert C. (2013). Phytoremediation: A Green Technology to Remove Environmental Pollutants f  herbs/2140.html herbs/2140.html  _29110.htm _29110.htm