Tubular Organs. Tracheal Development DT LT DB Dysfusion (Dys) is a bHLH-PAS Protein bHLHPAS-1PAS-2.

Slides:

Advertisements

Similar presentations

Control of Expression In Bacteria –Part 1

Advertisements

20,000 GENES IN HUMAN GENOME; WHAT WOULD HAPPEN IF ALL THESE GENES WERE EXPRESSED IN EVERY CELL IN YOUR BODY? WHAT WOULD HAPPEN IF THEY WERE EXPRESSED.

Genetic Analysis of Lac Operon Make partial diploids to do complementation tests: 1 copy of lac operon on E. coli chromosome. 2nd copy of lac operon on.

Regulation of Tracheal Fusion by Arf like GTPase Confusion DT DB LT GB.

Tre1 GPCR initiates germ cell transepithelial migration by regulating E-cadherin Prabhat S. Kunwar, Hiroko Sano, Andrew D. Renault, Vitor Barbosa, Naoyuki.

Promoters Information about where to start transcription.

Fusion cell gene expression profile Molecular mechanism of target gene regulation by Dys::Tgo Identify enhancer of dys targets: CG13196, CG15252 and mbo.

Fusion cell gene profiling Identify mbo and CG15252 enhancer Molecular mechanism of target gene regulation by Dys::Tgo Dys::Tgo activation of CG13196 Dys:Tgo.

Transcriptional regulation of tracheal fusion cell gene expression Isolate enhancer of three fusion cell expressed genes: dys, CG15252, CG13196 Identify.

E2A – bHLH transcription factor-fusion proteins in Leukemia

MDCK tubulogenesis can be divided into stages with different molecular requirements Requires ERK activation Requires MMP activity From O’Brienet al,

MCB 317 Genetics and Genomics MCB 317 Topic 10, part 3 A Story of Transcription.

Gene regulation  Two types of genes: 1)Structural genes – encode specific proteins 2)Regulatory genes – control the level of activity of structural genes.

Control of gene expression Unit but different cells have different functions and look and act differently! WHY? Different sets of genes are expressed.

Cells of E. coli bacteria Allow cells to respond to changes in their environment Only make proteins when they are needed.

Gene Expression and Regulation

Tubular Organs. From Uv et al, 2003 Drosophila Tracheal System Simple structure Powerful genetics Easy observation.

Gene repression and activation

AP Biology Control of Eukaryotic Genes.

Section 2 CHAPTER 10. PROTEIN SYNTHESIS IN PROKARYOTES Both prokaryotic and eukaryotic cells are able to regulate which genes are expressed and which.

How Does A Cell Know? Which Gene To Express Which Gene To Express& Which Gene Should Stay Silent? Which Gene Should Stay Silent?

Gene regulation. How does DNA make different cells? All cells have a full set of DNA Not all the DNA is expressed in each one Promoters and repressors.

The bHLH-PAS protein ARNT - effects on ER signalling.

Gene Expression. Remember, every cell in your body contains the exact same DNA… …so why does a muscle cell have different structure and function than.

Prokaryotic cells turn genes on and off by controlling transcription.

Biochemistry Sixth Edition Chapter 31 The Control of Gene Expression Part II: Eukaryotes (cis vs. trans) Copyright © 2007 by W. H. Freeman and Company.

Figure S1. Alignment of sequences from the 5′-end to the Sm binding site of reported genomic sequences (9-15) for HSUR 1. MicroRNA binding sites are.

GENE EXPRESSION. Pretty Please Back doorBig Brother.

G ENE R EGULATION. H OW DOES A CELL KNOW ? which gene to EXPRESS & which gene should stay SILENT?

Regulating Gene Expression To accompany “Regulating Gene Expression” Packet -review packet reading, pictures, and questions.

Control of Gene Expression

Trans-acting siRNA Brett Johnson BMS265.

Control of Gene Expression

Transcriptional Regulation

Prokaryotic cells turn genes on and off by controlling transcription.

Prokaryotic cells turn genes on and off by controlling transcription.

Chapter 12.5 Gene Regulation.

Daily Warm-Up January 8th

Daily Warm-Up Thursday, January 9th

Trachealess—A New Transcription Factor Target for PKB/Akt

A CRTCal Link between Energy and Life Span

Dan Gordon Gastroenterology Volume 114, Issue 4, (April 1998)

Eukaryote Regulation and Gene Expression

Control of Eukaryotic Genes

From Prescription to Transcription: Genome Sequence as Drug Target

Control of Gene Expression in Eukaryotic cells

Integrins and Mutant p53 on the Road to Metastasis

Cell to Cell Communication

Integrins and Mutant p53 on the Road to Metastasis

Prokaryotic cells turn genes on and off by controlling transcription.

Coordinate Transcriptional Regulation by ERG and Androgen Receptor in Fusion- Positive Prostate Cancers Yu Chen, Charles L. Sawyers Cancer Cell Volume.

SP1 was a downstream target of miR-150-3p

Gene Expression Activation of a gene to transcribe DNA into RNA.

Monday’s Writing Topic ~ Week 20 – January 25 – 29 ~

Comparison of Nuclear, Eukaryotic RNA Polymerases

Trachealess—A New Transcription Factor Target for PKB/Akt

Prokaryotic cells turn genes on and off by controlling transcription.

The Bare Lymphocyte Syndrome: Molecular Clues to the Transcriptional Regulation of Major Histocompatibility Complex Class II Genes Angela DeSandro, Uma.

Prokaryotic (Bacterial) Gene Regulation

Prokaryotic cells turn genes on and off by controlling transcription.

The Expanding Cosmos of Nuclear Receptor Coactivators

Alexander Kiani, Anjana Rao, Jose Aramburu Immunity

Prokaryotic cells turn genes on and off by controlling transcription.

Gene Regulation A gene (DNA) is expressed when it is made into a functional product (protein/enzyme)

Fig. 3 Conditionally expressed CAR using Notch as a signal induction and response pathway system. Conditionally expressed CAR using Notch as a signal induction.

Volume 119, Issue 4, Pages (October 2000)

Prokaryotic cells turn genes on and off by controlling transcription.

Heather M. Young, Lincon A. Stamp Gastroenterology

Opening Chromatin Molecular Cell

Presentation transcript:

Tubular Organs

Tracheal Development DT LT DB

Dysfusion (Dys) is a bHLH-PAS Protein bHLHPAS-1PAS-2

Dysfusion Is Expressed In Fusion Cells DT DB LT Btl-GAL4, UAS-lacZ Dys

dys Mutants dys 3 dys 2 bHLHPAS-1PAS-2 Mab 2A12 WTdys

How dys expression is regulated? How Dys:Tgo regulates down-stream target genes?

Dysfusion is Expressed in Mutiple Cell Types

Identify dys Enhancer

Identify dys Fusion Cell Enhancer

How dys expression is regulated? Is dys auto-regulated by Dys:Tgo? N283-GFP,dys/Tm3, twi-gal4, UAS-GFP C1.7-GFP, dys/TM3, twi-gal4, UAS-GFP Is dys regulated by esg? esg/cyo, twi-gal4, UAS-GFP; N283 esg/cyo, twi-gal4, UAS-GFP; C1.7 Identify other transcription factors binding sites in dys enhancer. Identify dys fusion cells enhancer in C1.7 and G1.7 Ongoing……

Summary of dys Function N TC GTG members only (Protein trafficking) Trachealess (Migration) CG13196, E-cadherin (cell adhesion) CG15252 (unknown function) Tgo Dys Tgo Dys Tgo Dys Identified dys Targets

Two TCGTG Sites are Required to Activates CG13196

Two TCGTG Sites of CG13196 are Conserved

Two TCGTG Sites are Required to Activates CG15252

Two TCGTG Sites of CG15252 are Conserved

Potential Co-activator of Dys:Tgo is Required to Turn on Gene Expression in Additional Fusion Cells

Ongoing..