Pectinase An Enzyme By Jack Carroll.

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Presentation transcript:

Pectinase An Enzyme By Jack Carroll

Pectinase is a very complex enzyme that is actually composed of three Abstract Pectinase is a very complex enzyme that is actually composed of three repeatedly active enzymes – they are pectinesterase, polygalacturonase, pectinlyase – which ensures a extremely high level of the decomposition of the pectin.

Pectin is a polysaccharide found in many fruits and some vegetables Pectin is a polysaccharide found in many fruits and some vegetables. The chemical name for pectin is polygalacturose.

SUBSTRATE ENZYME

Pectinase – A complex enzyme that breaks down pectin, a polysaccharide found in plant cell walls. Products: One of the enzymes products can be used to increase the yield of juice from apples, etc. and also to peel citrus and other fruits.

Optimal Temperature and PH Denaturation of Pectinase Pectinase (commercial) has about 64.4°F and 113° F for temp and works well at a pH of 4.5 to 5.5. Denaturation of Pectinase Pectinase will denature if the temperature is above 45 ° C, or 113° F. Pectinase will not denature in cold temperatures.

There are no cofactors or coenzymes for this enzyme. Inhibition of Pectinase: Common inhibitors of pectinase are tannin, glycine, and formaldehyde. Others are monoiodoacetic acid, mercuric chloride, sodium azide, sodium fluoride, ammonium arsenate, and sulfur dioxide, but pectinase is more resistant to those. Pectinase is destroyed by heat, heavy-metal salts, strong acids, and strong alkali's. There are no cofactors or coenzymes for this enzyme.

Pectinase - GMO Database. (n.d.). Retrieved from WORKS CITED: Pectinase - GMO Database. (n.d.). Retrieved from http://www.gmocompass.org/eng/database/enzymes/92.pectinase.html Pectinase Database - Department of Biotechnology MSRIT. (n.d.). Retrieved from http://pec.biodbs.info/ REACTIONS & ENZYMES. (n.d.). Retrieved from http://www2.estrellamountain.edu/faculty/farabee/biobk/biobookenzym.html The effect of catalysts on rates of reaction. (n.d.). Retrieved from http://www.chemguide.co.uk/physical/basicrates/catalyst.html Pectinase - an Enzyme Reaction Tutorial | Sophia Learning. (n.d.). Retrieved from http://www.sophia.org/tutorials/pectinase-an-enzyme-reaction Department of Food, Bioprocessing and Nutrition Sciences North Carolina State University. (n.d.). Pectinase Inhibition. Retrieved from http://fbns.ncsu.edu/USDAARS/Acrobatpubs/P59-89/p69.pdf Biovet. (n.d.). www.biovet.com. Retrieved from http://www.biovet.com/rus/pdfs/Pectinase.pdf