Biosynthesis of 生科系博士班 李曉青 資工系博士班 張君天 資工系博士班 林沿妊 Group 3

Powerful antioxidant, reduces the risk of –Coronary heart disease by preventing oxidation of low density lipoprotein (LDL) cholesterol –Atherosclerosis by protecting plasma lipids from oxidation –Oxidative damage of lymphocyte DNA –Cancer

Protein ID Protein nameGene deoxy-D-xylulose-5-phosphate synthase dxs E.coli C-methyl-D-erythritol 4-phosphate (MEP) synthasedxr E.coli C-methyl-D-erythritol 4-phosphate cytidylyltransferase ygbP E.coli (cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase ychB E.coli C-methyl-D-erythritol 2,4-cyclodiphosphate synthaseygbB E.coli hydroxy-3-methylbut-2-en-1-yl diphosphate synthasegcpE E.coli hydroxy-3-methylbut-2-enyl diphosphate reductaselytB E.coli isopentenyl-diphosphate delta-isomerase b2889E.coli farnesyl-diphosphate synthaseispA E.coli geranylgeranyl pyrophosphate synthetasecrtE E.uredovora phytoene synthetasecrtB E.uredovora oxidoreductase crtI E.uredovora zeta-carotene desaturasecrtQ E.uredovora

dxs:DXP synthase idi: IPP isomerase ispA: Farnesyl-PP synthase

crt E: GGPP synthase crt B: phytoene synthase crt I: lycopene synthase from E.uredovora gps:ggpp synthase from A.fulgidus

Red : E.coli Blue : A.fulgidus Green: E.uredovora

Wang CW, Oh MK, Liao JC. Engineered isoprenoid pathway enhances astaxanthin production in Escherichia coli. Biotechnol Bioeng Jan 20;62(2): Misawa N, Nakagawa M, Kobayashi K, Yamano S, Izawa Y, Nakamura K, Harashima K. Elucidation of the Erwinia uredovora carotenoid biosynthetic pathway by functional analysis of gene products expressed in Escherichia coli. J Bacteriol Dec;172(12): Farmer WR, Liao JC. Improving lycopene production in Escherichia coli by engineering metabolic control. Nat Biotechnol May;18(5): References