PCR. PCR AMPLIFICATION PCR APPLICATIONS b MOLECULAR BIOLOGY b ENVIRONMENTAL SCIENCE b FORENSIC/MEDICAL SCIENCE b BIOTECHNOLOGY b GENETICS b GENE CLONING.

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Presentation transcript:

PCR

PCR AMPLIFICATION

PCR APPLICATIONS b MOLECULAR BIOLOGY b ENVIRONMENTAL SCIENCE b FORENSIC/MEDICAL SCIENCE b BIOTECHNOLOGY b GENETICS b GENE CLONING b MICROBIOLOGY

DNA STRUCTURE b SUGAR-PHOSPHATE BACKBONE b A=ADENINE b T=THYMINE b C=CYTOSINE b G=GUANINE

PCR REQUIREMENTS b DNA b PRIMERS b dATP, dTTP, dCTP, dGTP b POLYMERASES

RAPD PCR b RANDOM b AMPLIFIED b POLYMORPHIC b DNA

RAPD APPLICATIONS b DNA FINGERPRINTING b ANALYSIS OF ANCIENT DNA FROM FOSSILS b MAPPING THE HUMAN GENOME b FINGERPRINTING UNKNOWN MICROORGANISMS

RAPD PRIMERS b 10 NUCLEOTIDES LONG b ARBITRARY SEQUENCE b BINDS AT MULTIPLE SEQUENCES ALONG DNA b NON-SPECIFIC BINDING b ACCURACY REQUIRES AT LEAST 10 PRIMERS