We have viruses!! Tucson High School Biotechnology Course Spring 2010.

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Presentation transcript:

We have viruses!! Tucson High School Biotechnology Course Spring 2010

Results from Last Class Synechococcus Prochlorococcus

Results from Last Class: Controls Negative Control Positive Control 10 4 dilution (higher concentration of viruses) Positive Control 10 6 dilution (lower concentration of viruses

Results from Last Class: Tara Oceans Samples No Plaques: 11 Tara Samples Plaques!! 6 Tara Samples

Results from Tara Oceans

Now What?? Pick Plaques! Yes! No! 3 μl

What’s Next? Generate many more viruses We need a lot! Each virus: ~55 ag DNA ( g) We need: a few μg ( g) => 1.8 x viruses ( viruses)

A reminder Overlays are NOT solid! **Do NOT tilt, shake, jostle, or turn over your plates!**