Plasmid mediated Metallo-Beta- Lactamase (MBL) Plasmid mediated Hydrolyses all beta-lactam (except aztreonam) Inhibited by EDTA Binds zinc (Zn 2+ ) Pseudomonas.

Slides:



Advertisements
Similar presentations
Detection of ESBLs & AmpC
Advertisements

Detection of b-Lactamase-Mediated Resistance
Emerging Antimicrobial Resistance in Texas The new ESBLs.
2009 CLSI M100-S19 Update Nebraska Public Health Laboratory.
Methods for detection β-lactamases Sarah Alharbi.
New Resistance in Gram Negative Rods (GNRs)
Klebsiella oxytoca and K1 enzyme
Welcome to the CDS Workshop Jeanette Pham Syd Bell Hobart 2011.
ESBL producing Pseudomonas aeruginosa resistant to CAZ, ATM, FEP, borderline resistant to PIP, susceptible to TIM and TZP. Note the synergy between TIM.
A review and update of the CDS test CDS Workshop ASM 2009 Perth.
WELCOME to the CDS WORKSHOP Sydney Excel Spreadsheet for Registration.
Plate 1a: Staphylococcus aureus resistant to penicillin (P0.5) only. Note the annular radius of the zone of inhibition of 9.5 mm around the cefoxitin (FOX.
Antimicrobial resistance surveillance in Ireland Results of invasive Pseudomonas aeruginosa infection (blood/CSF) surveillance, 2009 **** Data as of 01/12/2010.
n°10 : Klebsiella pneumoniae
Resistance to  -lactam antibiotics within the Enterobacteriaceae Paul D. Fey, Ph. D. University of Nebraska Medical Center.
COL Helen Viscount, PhD, D(ABMM) LTC Steven Mahlen, PhD, D(ABMM)
Beta lactam antibiotics & Other cell wall synthesis inhibitors
12 August 2003 AmpC  -Lactamases & their detection David Livermore Health Protection Agency, Colindale, London.
Clinical Cases Beta-Lactam Answers. Case 1 What antibiotic would you recommend for intravenous therapy in a 40yo BM with a Staphylococcus aureus (MSSA)
Antimicrobial resistance surveillance in Ireland Results of invasive Escherichia coli infection (blood/CSF) surveillance, 2009 **** Data as of 01/12/2010.
Detection of  -lactamase- mediated resistance David Livermore Health Protection Agency, Colindale, London.
ESBL, MBL and Amp C beta lactamase detection DEPARTMENT OF MICROBIOLOGY JIPMER PUDUCHERRY.
Resistant Gram-Negative Bacilli ESBLs and Other Bad Bugs David P. Dooley, FACP UTHSC-San Antonio Audie Murphy VA Hospital San Antonio, TX.
Interpretation/Evaluation of Laboratory Data CLS 552 Human Microbiology & Immunology Laboratory Note: View this PowerPoint as a ‘Full Screen Slide Show’.
김의종(집필대표), 강정옥, 김미나, 김민중, 김성일, 이경원, 이도현, 이장호, 이남용, 이창규, 신종희, 장철훈, 최혜심
Nosocomial E. coli Urine Isolates 1991 – Nosocomial K. pneumoniae Urine Isolates
Antimicrobial resistance surveillance in Ireland Results of invasive Klebsiella pneumoniae infection (blood/CSF) surveillance, 2009 **** Data as of 01/12/2010.
Nosocomial E. coli Urine Isolates Nosocomial K. pneumoniae Urine Isolates
Dr. Laila M. Matalqah Ph.D. Pharmacology
ESBL, AmpC -lactamase 표준진단법
(Ambler class A, Bush group 2) Inhibited by CA
4/28/2017 AST.
MRSA, ESBLs and Carbapenem Resistance
C-158 Genetic and Biochemical Characterisation of OXA-405, a New Extended Spectrum Class D -Lactamase from Serratia marcescens L. Dortet1,2, S. Oueslati1,
Cephalosporins Four Generations! Bactericidal & Time-Dependent!
Antimicrobial Resistance at GLA
Current Status of Antimicrobial Resistance
N°XXXX Rapid detection of extended-spectrum BETA-lactamase producing Enterobacteriaceae from urine using the ESBL NDP test Patrice Nordmann, Laurent Poirel,
Serotypes, genotypes and antimicrobial resistance patterns of human diarrhoeagenic Escherichia coli isolates circulating in southeastern China  Y. Chen,
EARS-Net data on Antimicrobial Resistance in Ireland, Q
Standardisation – What does this mean for the CDS?
Alphabet Soup: MRSA – ESBL - CRE
International Journal of Infectious Diseases
The Role of the Microbiology Laboratory in AMS programs
Antimicrobial Spectrum of Activity Visual Learning Exercises (“Flower Diagrams”) This work is licensed under the Creative Commons Attribution-NonCommercial-
Cell wall inhibitor Cephalosporins Dr. Naza M. Ali Lec D
F.M. MacKenzie, C.A. Miller, I.M. Gould 
Emergence of Klebsiella pneumoniae with an AmpC(DHA-1) and blaSHV-11 in a Belgian hospital. Timothy Vanwynsberghe1, Katia Verhamme2, Marijke Raymaekers3,
Clinico-Pathological Conference (CPC) Meet
Antibiotics sensitivity of microorganism causing nosocomial infections
Metallo-β-lactamase-producing Pseudomonas aeruginosa in the Netherlands: the nationwide emergence of a single sequence type  A.K. Van der Bij, D. Van.
D-735/178 50th ICAAC Sept , 2010 Boston
Table 1: Resistance profile
C th Interscience Conference on Antimicrobial Agents and Chemotherapy October 25-28, Washington, DC Examining Temocillin Activity in Combination.
L. Poirel, P. Nordmann  Clinical Microbiology and Infection 
C th Interscience Conference on Antimicrobial Agents and Chemotherapy October 25-28, Washington, USA Emergence of VIM-2 Metallo--lactamase.
Impact of changes in CLSI and EUCAST breakpoints for susceptibility in bloodstream infections due to extended-spectrum β-lactamase-producing Escherichia.
A multicentre analysis of epidemiology of the nosocomial bloodstream infections in Japanese university hospitals  M. Nagao  Clinical Microbiology and.
EXTENDED SPECTRUM β-LACTAMASE PRODUCING GRAM NEGATIVE BACTERIA ISOLATED FROM INFECTED WOUND OF ORTHOPEDIC PATIENTS Shwe Phyu Aung , Thandar Win Maung,
Evaluation of a diagnostic flow chart for detection and confirmation of extended spectrum β-lactamases (ESBL) in Enterobacteriaceae  S. Polsfuss, G.V.
Phenotypic detection of extended-spectrum β-lactamase production in Enterobacteriaceae: review and bench guide  L. Drieux, F. Brossier, W. Sougakoff,
L.-T. Wu, S.-W. Hung, Y.-C. Chuang, H.-E. Chen, R.N. Jones, W.-L. Yu 
Β-LACTAM ANTIBIOTICS THIENAMYCIN MONOBACTAMS β-LACTAM INHIBITORS.
I. Faria-Ramos, M. J. Espinar, R. Rocha, J. Santos-Antunes, A. G
Highly antibiotic-resistant Acinetobacter baumannii clinical isolates are killed by the green tea polyphenol (–)-epigallocatechin-3-gallate (EGCG)  A.
Jacques Sirot  Clinical Microbiology and Infection 
Transferable plasmid mediating resistance to multiple antimicrobial agents in Klebsiella pneumoniae isolates in Greece  I. Galani, E. Xirouchaki, K. Kanellakopoulou,
Modified Hodge and EDTA-disk synergy tests to screen metallo-β-lactamase-producing strains of Pseudomonas and Acinetobactet species  K. Lee, Y. Chong,
Multi-centre evaluation of a phenotypic extended spectrum β-lactamase detection guideline in the routine setting  T.N. Platteel, J.W. Cohen Stuart, A.J.
Serotypes, genotypes and antimicrobial resistance patterns of human diarrhoeagenic Escherichia coli isolates circulating in southeastern China  Y. Chen,
Presentation transcript:

Plasmid mediated Metallo-Beta- Lactamase (MBL) Plasmid mediated Hydrolyses all beta-lactam (except aztreonam) Inhibited by EDTA Binds zinc (Zn 2+ ) Pseudomonas aeruginosa or Enterobacteriaceae

MBL producing Pseudomonas aeruginosa. The isolate is resistant to imipenem (IPM 10) ceftazidime (CAZ 10), tazocin (TZP 55), cefepime (FEP 10), Timentin (TIM 85), and susceptible to aztreonam (ATM 30).

MBL producing Pseudomonas aeruginosa. The blank disc contains 750 μg EDTA. The zone of inhibition of imipenem (IPM 10) and meropenem (MEM 5) is increased with the addition of EDTA indicating the presence of a Metallo-Beta- Lactamase.

MBL producing Escherichia coli resistant to cefotaxime (CTX 5), cefotetan (CTT 30), Augmentin (AMC 60), and susceptible to aztreonam (ATM 30). The blank disc contains 750 μg EDTA. Note the borderline resistance to imipenem (IPM 10) and the large zone around the IPM disc loaded with 750 μg EDTA.

MBL producing Klebsiella pneumoniae with multiple resistance: R/ imipenem (IPM 10), cefotaxime (CTX 5), cefotetan (CTT 30), and Augmentin (AMC 60). The resistance to aztreonam (ATM 30) is due to the presence of an ESBL (key hole). Note the borderline resistance to IPM and the large zone around the IPM disc loaded with 750 μg EDTA.

Beta-Lactamases vs Beta-Lactams P/CFEPCTTATM MBLPRRS ESBLPRSR AmpCP/CSRS/R P = Plasmid, C = Chromosomal