LUCIFERASE A. Cengiz Öztireli. BIOLUMINESCENCE BIOLUMINESCENCE (LIVING LIGHT) Milky Sea.

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Presentation transcript:

LUCIFERASE A. Cengiz Öztireli

BIOLUMINESCENCE BIOLUMINESCENCE (LIVING LIGHT) Milky Sea

BIOLUMINESCENCE Applications

BIOLUMINESCENCE Sea AnemonesBacteriaDinoflagellates

BIOLUMINESCENCE FIREFLYES (PHOTINUS) Attracting mates Attracting prey

LUCIFERASE FIREFLY BACTERIA

BIOLUMINESCENCE 8 ATP → 1 Green Photon Reaction: Luciferin Luciferase + O 2 Inter.P*P* P + hv ATP

BIOLUMINESCENCE Color  P *  P * + F → F * + P  Proteins around  Structure

PHOTINUS PYRALIS (FIREFLY)

PHOTINUS PYRALIS LUCIFERASE Luciferase + Luc-COOH ATP Luciferase: Luc-CO-AMP O2O2 Luciferase: Luc=O *

PHOTINUS PYRALIS LUCIFERASE N-Terminal C-Terminal Cleft Structure and Functioning

CONCLUSIONS THANK YOU

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