Sox2 : Oct-3/4 partnership. Sox2 and Oct-3/4 work together cooperatively to regulate their own transcription and the transcription of a large set of downstream.

Slides:

Advertisements

Similar presentations

Andrea Hand BIOL 303. Large Intergenic Non-Coding RNAs Intergenic Non-Coding: transcribed from a region of DNA between two genes that are used to make.

Advertisements

Genome-wide mapping of transcription factor Oct4, Sox2 and Nanog binding-sites in mouse embryonic stem cells Genome Institute of Singapore Department of.

Self-renewal vs. Differentiation of Mouse Embryonic Stem Cells TVGH Journal Club, introduced by Dr. 張宏吉, May 6, K. Sue O'Shea 2004 Biology of Reproduction.

Extracellular Matrix Protein Anosmin Promotes Neural Crest Formation and Regulates FGF, BMP, and WNT Activities Yukinori Endo, Hiroko Ishiwata-Endo, Kenneth.

CHAPTER 10 DATA COLLECTION METHODS. FROM CHAPTER 10 Copyright © 2003 John Wiley & Sons, Inc. Sekaran/RESEARCH 4E.

Stem cell biology basics By the end of this lecture you will: 1.Understand where stem cells come from 2.Understand the different types of stem cells 3.Understand.

Human long non ‐ coding RNAs promote pluripotency and neuronal differentiation by association with chromatin modifiers and transcription factors by Shi.

Volume 17, Issue 2, Pages (August 2015)

Generation of Induced Pluripotent Stem Cell Lines from Adult Rat Cells

FoxO: A New Addition to the ESC Cartel

Volume 137, Issue 4, Pages (May 2009)

Volume 4, Issue 6, Pages (June 2009)

Establishment of Endoderm Progenitors by SOX Transcription Factor Expression in Human Embryonic Stem Cells Cheryle A. Séguin, Jonathan S. Draper, Andras.

Volume 11, Issue 2, Pages (August 2012)

T.S. Karin Eisinger-Mathason, M. Celeste Simon Cancer Cell

Patient-Specific Naturally Gene-Reverted Induced Pluripotent Stem Cells in Recessive Dystrophic Epidermolysis Bullosa Jakub Tolar, John A. McGrath, Lily.

Wing Y. Chang, William L. Stanford Cell Stem Cell

Unraveling the Pros and Cons of Interferon-γ Gene Regulation

A Precarious Balance: Pluripotency Factors as Lineage Specifiers

Volume 9, Issue 5, Pages (November 2017)

Volume 5, Issue 3, Pages (September 2015)

Jan Philipp Junker, Alexander van Oudenaarden Molecular Cell

Volume 17, Issue 4, Pages (October 2015)

Volume 26, Issue 3, Pages (March 2007)

Claire A. Higgins, Munenari Itoh, Keita Inoue, Gavin D

Rudolf Jaenisch, Richard Young Cell

Volume 17, Issue 2, Pages (August 2015)

Volume 55, Issue 4, Pages (August 2014)

Volume 7, Issue 1, Pages (July 2010)

Volume 6, Issue 4, Pages (April 2010)

Shi-Yan Ng, Gireesh K. Bogu, Boon Seng Soh, Lawrence W. Stanton

Novel p53 target genes identified by RNA-Seq, pSILAC and ChIP-Seq.

Silencing Insulin Resistance through SIRT1

Volume 21, Issue 3, Pages e7 (September 2017)

The Noncanonical Binding Site of the MED-1 GATA Factor Defines Differentially Regulated Target Genes in the C. elegans Mesendoderm Gina Broitman-Maduro,

Meddling with METTLs in Normal and Leukemia Stem Cells

Volume 46, Issue 1, Pages (April 2012)

Volume 7, Issue 4, Pages (October 2016)

Wei Jiang, Yuting Liu, Rui Liu, Kun Zhang, Yi Zhang Cell Reports

Volume 18, Issue 4, Pages (April 2010)

A Penetrating Look at Stochasticity in Development

Gene expression profiling of human bone marrow-derived mesenchymal stem cells during adipogenesis DOI: /FHC.a Gene-act-network according.

Volume 49, Issue 1, Pages 1-3 (January 2013)

Volume 132, Issue 6, Pages (March 2008)

Volume 122, Issue 6, Pages (September 2005)

Nanog Cell Volume 113, Issue 5, Pages (May 2003)

Volume 12, Issue 12, Pages (September 2015)

Volume 13, Issue 3, Pages (September 2013)

Volume 58, Issue 2, Pages (April 2015)

Volume 55, Issue 5, Pages (September 2014)

Targeting SOX17 in Human Embryonic Stem Cells Creates Unique Strategies for Isolating and Analyzing Developing Endoderm Pei Wang, Ryan T. Rodriguez,

Volume 55, Issue 5, Pages (September 2014)

Volume 7, Issue 1, Pages (July 2010)

SQUAMOSA Promoter Binding Protein-like Transcription Factors: Targets for Improving Cereal Grain Yield Qian Liu, Nicholas P. Harberd, Xiangdong Fu Molecular.

Jakub Tolar, Lily Xia, Megan J. Riddle, Chris J. Lees, Cindy R

Volume 6, Issue 3, Pages (March 2016)

Epigenetics of Reprogramming to Induced Pluripotency

Regulatory Principles of Pluripotency: From the Ground State Up

Volume 9, Issue 5, Pages (November 2017)

Regulation of developmental pathways by core transcription factors via their target genes in the EpiSC model. Regulation of developmental pathways by core.

Model of Eomes action in gastrulation.

Genetic and Epigenetic Regulators of Pluripotency

Oct4: The Final Frontier, Differentiation Defining Pluripotency

Capturing Pluripotency

Abby Sarkar, Konrad Hochedlinger Cell Stem Cell

Pax6 Is a Human Neuroectoderm Cell Fate Determinant

Volume 2, Issue 3, Pages (March 2008)

The Molecular Harbingers of Early Mammalian Embryo Patterning

Opening Chromatin Molecular Cell

Yasuhiro Yamada, Hitomi Aoki, Takahiro Kunisada, Akira Hara

Presentation transcript:

Sox2 : Oct-3/4 partnership. Sox2 and Oct-3/4 work together cooperatively to regulate their own transcription and the transcription of a large set of downstream target genes in EC and ES cells. A Rizzino WIREs Syst Biol Med DOI: /wsbm.012 Copyright © 2009 John Wiley & Sons, Inc.

Consensus sequence for HMG/POU cassettes. A consensus sequence for HMG/POU cassettes derived by comparing the HMG/POU sequences of six Oct-3/4:Sox2 target genes (FGF-4, Sox2, Oct-3/4, Nanog, Fbx15, and UTF1). The triangle represents possible inserts of up to three base pairs between the HMG and POU motifs. For example, the FGF-4 gene contains a three-base-pair insert between its HMG and POU motifs. For the remaining five genes, as well as their HMG and POU motifs, are directly adjacent to one another. A Rizzino WIREs Syst Biol Med DOI: /wsbm.012 Copyright © 2009 John Wiley & Sons, Inc.

An expanded model for the regulation of Oct-3/4:Sox2 target genes. Sox2 and Oct-3/4 work together cooperatively to regulate their own transcription and the transcription of a large set of downstream target genes. This includes genes expressed in ES cells and those not expressed in ES cells. A Rizzino WIREs Syst Biol Med DOI: /wsbm.012 Copyright © 2009 John Wiley & Sons, Inc.

Sox protein redundancy and the regulation of Oct-3/4 : Sox target genes. Oct-3/4 is proposed to work in conjunction with Sox2, as well as Sox4, Sox11, and Sox15, in ES cells to regulate the transcription of the Oct-3/4 and Sox2 genes as well as the transcription of a large set of downstream target genes. Sox2 is also proposed to indirectly regulate the transcription of the Oct-3/4 gene by upregulating Nr5a2 and downregulating Nr2f2, which are positive and negative regulators of the Oct-3/4 gene, respectively. As indicated by the dotted lines, it remains to be determined whether Sox4, Sox11, or Sox15 influence the expression of the Oct-3/4 and Sox2 genes. A Rizzino WIREs Syst Biol Med DOI: /wsbm.012 Copyright © 2009 John Wiley & Sons, Inc.

Elevating the expression of Sox2 reducing the expression of genes required for the self-renewal of ES cells. Elevating the levels of Sox2 in EC and ES cells reduces the expression of the Oct-3/4 gene and the expression of downstream Sox : Oct target genes, such as FGF-4, Nanog, and UTF1. As indicated by the dotted lines, it is unclear whether Oct-3/4 continues to positively influence the expression of Oct-3/4 : Sox2 target genes when Sox2 is overexpressed. A Rizzino WIREs Syst Biol Med DOI: /wsbm.012 Copyright © 2009 John Wiley & Sons, Inc.

Oct-3/4 and Sox2 function as molecular rheostats. Increasing or decreasing the levels of either Sox2 or Oct-3/4 in ES cells promotes their differentiation. However, the spectra of cell types that form vary. The cell types formed were determined by markers expressed in the differentiated cell populations. TE, trophectoderm; Endo, endoderm; Meso, mesoderm; ECTO, ectoderm. A Rizzino WIREs Syst Biol Med DOI: /wsbm.012 Copyright © 2009 John Wiley & Sons, Inc.