PCR Overview Lifted from various powerpoint presentations on the internet.

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Research Techniques Made Simple: Polymerase Chain Reaction

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Presentation transcript:

PCR Overview Lifted from various powerpoint presentations on the internet

PCR (Polymerase Chain Reaction) Invented by Dr. Kary Mullis in 1983 In vitro amplification of specific DNA fragment Uses two oligonucleotide primers complementary to opposite strands of a duplex DNA. Taq DNA polymerase –Thermus aquaticus Thermostable polymerases

Invented by Kary Mullis Mullis and Faloona, Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction. Nobel Prize 1993

PCR DNA replication of a desired region of the genome in a test tube.

Templates for PCR

Determine your target region

PCR Animation

PCR strip tubes

Thermal Cycler

Gel Electrophoresis