Sudeep Perumbakkam Oregon State University. What is remediation?  Hazardous waste is everywhere.  It comes from paints, motor oil, hair spray, household.

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Presentation transcript:

Sudeep Perumbakkam Oregon State University

What is remediation?  Hazardous waste is everywhere.  It comes from paints, motor oil, hair spray, household cleaners, automotive chemicals, and all kinds of toxic medical, industrial and military products.

INCINERATION- Texas 1999

COST OF INCINERATION

BIOREMEDIATION Bioremediation is a complete, safe and natural process of cleaning up organic contaminants through the use of microbes.

TYPES OF REMEDIATION

IDEA

Agricultural solution to keeping ranges open

TNT Contamination problem

Pilot Greenhouse Study Fescue in sand, loam and clay Plants are treated with a solution of 14 C-TNT Treated plants were removed from the soil  7 days after dosing. SandLoamClay Sand Loam Clay

Cuttings of foliage from grass grown in soil treated with 14 C-TNT SandLoamClaySandLoamClaySandLoamClay 11 days after treatment with 14 C-TNT 27 days after treatment with 14 C-TNT 47 days after treatment with 14 C-TNT

Cuttings of foliage from grass grown in soil treated with 14 C-TNT (continued) SandLoamClaySandLoamClay 69 days after treatment with 14 C-TNT 89 days after treatment with 14 C-TNT

Approximate excretion of 14 C-TNT in sheep Liver Kidney Muscle Fat Body tissues: 17-19% Urine: 6-8% Feces: 75% Liver Kidney Muscle Fat Body tissues: 17-19% Urine: 6-8% Feces: 75% Liver Kidney Muscle Fat Body tissues: 17-19% Urine: 6-8% Feces: 75% Liver Kidney Muscle Fat Body tissues: 17-19% Urine: 6-8% Feces: 75% Liver Kidney Muscle Fat Body tissues: 17-19% Urine: 6-8% Feces: 75%

Sheep – ruminant model

Anaerobic transformation of 2,4,6-TNT by bovine ruminal microbes T.J. Fleischmann et al. (2004) Biochemical and Biophysical Research Communications 314:957–963 Proposed Transformation Pathway of 2,4,6-Trinitrotoluene by Rumen Microbes Under Anaerobic Conditions