16S rDNA phylotype distribution in libraries obtained from total RNA isolated from Lake Washington sediment and from 13 C-labelled and 12 C-DNA fractions.

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16S rDNA phylotype distribution in libraries obtained from total RNA isolated from Lake Washington sediment and from 13 C-labelled and 12 C-DNA fractions obtained from the microcosm experiments Phylogenetic groupPhylotypes LW RNA Methanol MethylamineFormaldehyde Formate 13 C DNA 12 C DNA 13 C DNA 12 C DNA 13 C DNA 12 C DNA 13 C DNA 12 C DNA Proteobacteria Betaproteobacteria pLW-1, pLW-2, pLW-3, pLW Comamonadaceae pLW-5, pLW Oxalobacteraceae pLW-7, pLW-8, pLW-9, pLW Nitrosomonadaceae pLW-11, pLW-12, pLW Methylophylaceae pLW-16 1 Gammaproteobacteria Methylobacter/Methylomonas pLW-17, pLW-18, pLW-19, pLW-20, pLW-21, pLW Pseudomonas pLW-23, pLW Achromatium pLW-25 2 Xanthomonadaceae pLW-26, pLW-27 5 Thioploca/Beggiatoa pLW-28, pLW-29 5 Uncl. Gammaproteobacteria pLW-30, pLW-31, pLW Alphaproteobacteria Caulobacterales pLW-33 1 Rhodobacterales pLW-34 1 Sphingomonadales pLW-35, pLW-36, pLW Uncl. Alphaproteobacteria pLW-38, pLW-39, pLW-40 2

Deltaproteobacteria Syntrophus pLW-41 1 Polyangiaceae pLW-42 1 Myxococcaceae pLW-43 3 Geobacteraceae pLW-44 1 Uncl. Deltaproteobacteria pLW-45, pLW-46, pLW Bacteroidetes Flexibacteraceae pLW-48 2 Saprospiraceae pLW-49 2 Sphingobacteriaceae pLW-50, pLW-51 3 Uncl. Bacteroidetes pLW-52, pLW-53, pLW-54, pLW-55, pLW-56, pLW Acidobacteria Holophaga/Geothrix pLW-58, pLW-59, pLW-60, pLW-61, pLW-62, pLW Uncl. Acidobacteria pLW-64, pLW Nitrospirae ncl. Nitrospira pLW-66, pLW-67, pLW WS3 pLW-69, pLW Actinobacteria Acidimicrobiaceae pLW-71, pLW Rubrobacteraceae pLW-73, pLW-74, pLW Uncl. Actinobacteria pLW-76, pLW

Gemmatimonadetes Gemmatimonadaceae pLW-78, pLW-79, pLW-80, pLW-81, LW-82, pLW-83, pLW-84, pLW-85, pLW-86, pLW Verrumicrobia Uncl. Verrumicrobia pLW-88, pLW-89 5 Planctomycetes Uncl. Planctomycetes pLW-90, pLW Chlorobi Uncl. Chlorobi pLW-92, pLW Spirochaetes Leptospiraceae pLW-94 1 Chloroflexi Uncl. Chloflexi pLW-95, pLW-96, pLW Fibrobacteres Uncl. Fibrobacteres pLW-98 1 Uncl. Bacteria pLW-99 3 pLW pLW pLW pLW pLW pLW pLW Total

a Classification according to the Ribosomal Database Project-II ( b Phylotypes were defined as sequences less than 97% similar (41). Uncl., phylotypes that could not be assigned with a bootstrap confidence estimate above the default threshold value of the RDP Classifier (80%) are displayed under an artificial ‘unclassified’ taxon