Light Microscopy Uses Uses Before you go to the EM Before you go to the EM Quick check Quick check Maybe all you need Maybe all you need Hydrated and living.

Slides:

Advertisements

Similar presentations

Chapter 4 Companion site for Light and Video Microscopy Author: Wayne.

Advertisements

Adjusting a Microscope 1Center components on optic axis 2Focus objective 3Focus condenser 4Adjust illumination lamp voltage (intensity) iris diaphragm.

Microscopes SC.912.L14.4.

Microscope Basics.

POWERPOINT PRESENTATION ON POLARISED MICROSCOPE

02 Dissecting Microscope. A B Carrying a Microscope.

Blizard Advanced Light Microscopy club Making friends with your microscope.

Single Lens Imaging System. Figure 29.8B Retina Optic nerve Fovea Optic nerve fibers Retina Photoreceptors NeuronsConeRod.

Biology 177: Principles of Modern Microscopy Lecture 08: Contrast and Resolution.

Stem Cell Instrumentation Foundry (SCIF) Orientation: Microscopy Venu Polineni Research Associate, SCIF University of California, Merced Phone: (559)

Microscopy Boot Camp /08/25 Nikitchenko Maxim Baktash Babadi.

Optics. Thomas Young ( ) Wave Optics Diffraction & Interference Christiaan Huygens ( )

Practical Aspects See: 1) anatomy/anatomy.htmlhttp://micro.magnet.fsu.edu/primer/ anatomy/anatomy.html 2)

Microscopes. Contents History Structure Uses Images.

Simple Microscope Objective magnification Working Distance Eyepiece magnification

USE AND CARE OF THE MICROSCOPE LECTURE 1. MICROSCOPY u Light Microscopy: any microscope that uses visible light to observe specimens u Compound Light.

Light Microscopy Sarah Heintz. Compound Microscope The microscope uses a lens that is close to the object and uses light to focus on the real image of.

Introduction to Microscopy. Objectives Learn to use a compound microscope correctly. Diagram the path of light through a compound microscope. Name major.

The Microscope.

A stereo dissecting microscope is normally used to view relatively large specimens at magnifications from about 5X to about 50X. The scope provides a very.

TOPIC: Microscope AIM: What are the parts of a microscope?

OU NanoLab/NSF NUE/Bumm & Johnson Microscopy & Resolution Magnification:Image size/Object size Resolution:The fineness of detail that can be distinguished.

PARTS OF THE MICROSCOPE

Parts of the Microscope and Their Function On the next slide there is an image of a microscope, very similar to the scopes we use in class. Click on the.

FNI 2B OM 1 Optical Microscopes. FNI 2B OM2 Outline Justification History Components of the Optical Microscope Theory of operation  Basic Microscope.

DIC Differential Interference Contrast Kim Peifley 08/14/15.

Agenda How to make the specimen visible – Definition of Contrast

By: Rob Page and Tara Trovarello

Geometric Optics. An object inside the focus casts a virtual image that is only focused by the eye.

WHAT IS A MICROSCOPE? A microscope is a device that uses lenses and light to magnify very small things in order to make them appear to be larger.

The Microscope. There are 2 types of microscopes: 1. Simple- contains one lens 2. Compound- contains 2 or more lenses.

Basic Microscopy – An Overview – October 2005 Protistology Course MBL, Woods Hole, MA.

Chapter 6 Companion site for Light and Video Microscopy Author: Wayne.

Parts of the Microscope Notes. Arm: This part supports the entire upper portion of the microscope.

Big Question: How do organisms interact with their environment? Goal: How do scientists study “invisible” interactions among organisms?

Contrast Microscopy Koehler Illumination Dark Field Phase Contrast Differential Interference Contrast Hoffman Modulation.

Thin-lens equation: 1/f = 1/d 0 + 1/d i. Magnification equation: h i /h o = d i /d o.

USING A MICROSCOPE. MICROSCOPE – TOOL USED TO MAGNIFY OR ENLARGE OBJECTS MAYBE MONOCULAR(1) OR BINOCULAR(2) EYEPIECES (MAGNIFIES 10 x) LOW-POWER OBJECTIVES.

Microscope Parts and Functions 1.Eyepiece – the part you look through 2.Course Adjustment Knob – the large knob which, when turned, brings the image of.

Chapter 7 THE MICROSCOPE.

Microscopes Biology I. Magnification 1. Power a microscope has to increase an objects size.

7-1 ©2011, 2008 Pearson Education, Inc. Upper Saddle River, NJ FORENSIC SCIENCE: An Introduction, 2 nd ed. By Richard Saferstein THE MICROSCOPE Chapter.

Break Period – move to lab

How a Microscope Works!. What Do You Do?!?! As we go through the diagram showing how a microscope works, draw the pictures and write the notes down on.

Microscopes. Compound Light Microscope Eyepiece--1 Body tube--2 Arm--3 Nosepiece--4 Stage clips--5 Objectives--6 Stage stop--7 Aperture--8 Stage--9 Coarse.

Microscopes…... Types….. 1 – Compound Light 2 – Transmission Electron (TEM) 3 – Scanning Electron (SEM)

The Microscope. The Basics An object is placed under the lower of two lenses, called the objective lens, it is viewed through the eyepiece lens.

Optical microscopy We want to see more details Magnification Resolution Contrast Simple microscope MENA3100, 26/1-10.

C.K.PITHAWALA COLLEGE OF ENG. &TECH. 1) PATEL DWIJ( ) 2) PATEL NISHIR J. ( ) 3) PATEL MILAN ( ) 4) JADAV ANAND ( )

Brightfield Contrasting Techniques Kurt Thorn Nikon Imaging Center University of California, San Francisco USA.

Microscope: instrument that magnifies small objects that cannot be seen by naked eye. Microscope: instrument that magnifies small objects that cannot.

Introduction to Light Microscopy

(Image: T. Wittman, Scripps) Introduction to Light Microscopy.

MICROSCOPE PARTS.

Meterm1 decimeterdm10 −1 m0,1 m1/10 m centimetercm10 −2 m0,01 m1/100 m millimetermm10 −3 m0,001 m1/1 000 m micrometer (or micron)μm10 −6 m0, m1/1.

Brightfield Contrasting Techniques Kurt Thorn NIC.

Parts of the Microscope and Their Function

The Microscope Micro – small Scope – to look at

Setting up a trinocular compound microscope for best viewing

Optical Instruments Or Seeing is Believing.

3.3 Other types of microscopy

Microscope Review.

Microscope Basics.

Microscope Review.

Microscope Basics.

Presentation transcript:

Light Microscopy Uses Uses Before you go to the EM Before you go to the EM Quick check Quick check Maybe all you need Maybe all you need Hydrated and living materials Hydrated and living materials

Light Microscopy Systems Systems Parts Parts Illumination Illumination Sample stage Sample stage Imaging Imaging

Light Microscopy Parts of the light microscope Parts of the light microscope Stand Stand Illuminator Illuminator Stage Stage Objective(s) Objective(s) Tube Tube Eyepiece(s) Eyepiece(s) Eye, camera Eye, camera

Light Microscopy Illumination Illumination Through the sample Through the sample Incident on surface of sample Incident on surface of sample

Light Microscopy Through sample illumination Through sample illumination Kohler illumination Kohler illumination Open field and condenser diaphragm Open field and condenser diaphragm Focus sample Focus sample Close field diaphragm and focus with condenser focus control. Use centering controls to center image. Close field diaphragm and focus with condenser focus control. Use centering controls to center image. Open field diaphragm to fill image Open field diaphragm to fill image Take out eyepiece and adjust condenser aperture to ¾ fill BFP of objective Take out eyepiece and adjust condenser aperture to ¾ fill BFP of objective

Light Microscopy Incident Light illumination Incident Light illumination Use special objectives Use special objectives Almost setup for Kohler illumination Almost setup for Kohler illumination Objectives serve as condenser too Objectives serve as condenser too

Light Microscopy Objectives Objectives Achromats- RB chromatic and G spherical corrected Achromats- RB chromatic and G spherical corrected Semi-apochromats- RBG chromatic + BG spherical Semi-apochromats- RBG chromatic + BG spherical Apochromats-RBBG chromatic + BG spherical Apochromats-RBBG chromatic + BG spherical “Plan” objectives correct for field curvature “Plan” objectives correct for field curvature

Light Microscopy Special Techniques Special Techniques Darkfield (hollow cone illumination) Darkfield (hollow cone illumination)

Light Microscopy Special Techniques Special Techniques Phase contrast (blocking phase object interactions with illumination) Phase contrast (blocking phase object interactions with illumination)

Light Microscopy Special Techniques Special Techniques Polarized light (polarized light interaction with birefringent materials) Polarized light (polarized light interaction with birefringent materials)

Light Microscopy Special Techniques Special Techniques DIC (Nomarski) (shearing interferometry) DIC (Nomarski) (shearing interferometry)