Nasonia vitripenni Jewel Wasp You are the PI (Primary Investigator)

Life Cycles

Haplo/Diplo Genetics

Wasp Development Timelines 18 o, 28 o C

Complementation Process by which an allele of each of two mutant genes makes up for the defect in the other gene. Process by which an allele of each of two mutant genes makes up for the defect in the other gene.

You Find Three Mutants With a Similar Phenotype Are they the Same Genotype?

Possible Physiology Enzyme A Enzyme B Enzyme C Enzyme A Enzyme B Enzyme C Enzyme A Enzyme B Enzyme C Enzyme A Enzyme B Enzyme C transport

Complement a||Ba||B a||Ba||B A||bA||b A||bA||b x A||bA||b a||Ba||B Two genes Both Recessive Think about dominant mutations!

Fail to Complement A||bA||b A||bA||b A||bA||b A||bA||b x A||bA||b A||bA||b 1 gene

Three scarlet eyed mutants, how many loci? st DR, st C, st 318 Connect same gene with a line. st DR st C st 318 Fail to complement For example. st D Hypothetically

Handling Wasps

Sexing Wasps adults

Sexing Wasps larvae, tuxedo – black stages

Sex Pictures

Scoring Red Eyes Here

Scoring Eye Color

Another Chart, Same Info

Wasp Business Link

To Do Plan experiment! Plan experiment!  define symbols, diagram crosses, determine expectations, etc. Each student : complementation. Each student : complementation. Per bench, or per pair (will tell next week), Per bench, or per pair (will tell next week),  recessive, dominance,  controls (stock maintenance). To Do Tuesday -Tuesday? …up to you!

Plan Your Crosses Think about the wasp’s schedule, Think about your schedule, Plan accordingly!

st DR, st C, st 318 F1 data, F2 data that means something