Rectifier Thermo siphon Reboiler To recycle water tank Degasser Hammer mills Slurry Tank Steam Jet Cook Tube Flash Tank Liquefaction tank Plate & Frame HX Beer Well Yeast Propagation Tank(2) Fermentation Tank(4) Stripper Column Rectifier Column CWR Centrifuges To dryers or wet feed Thin Stillage Tank 200 Proof Product Stripper Column Reboiler 4 Bottle molecules sieve system To syrup tank Dry-Milling Facility

Centrifuge Thin Stillage Evaporator Syrup Beer Bottom (Whole Stillage) To Cooker Wet Distillers Grain (WDG) Dryer Dried Distillers Grain with Solubles (DDGS) Stripper Column Byproduct Alternative Uses Oil Extraction Protein Extraction Fiber Separation Oil Proteins Soluble Fiber Biodiesel Animal supplement Industrial polymers Human dietary food Industrial polymers Alternative Uses Pretreatment Ethanol production Pure cellulsic material Lignin

Oil Extraction Beer Bottom Blender or Grinder Mixing Solvent (Hexane) Centrifuge Substrates for Protein Extraction Solvent Evaporation Top Liquid Phase (Solvent / Oil) Oil

Oil Extraction - Composition Oil Extraction Hexane (Hexane : Beer Bottom = 3 : 10) Beer Bottom (BB) Moisture : % Oil : % Protein : % Lignin : 7.32 % Starch : % Cellulose : % Hemicellulose : % Other : 4.14 % Oil Defatted BB Moisture : % Protein : % Lignin : 8.26 % Starch : % Cellulose : % Hemicellulose : % Other : 4.67 %

Protein Extraction : Scheme I Centrifuge Defatted BB Albumin Isolation Albumin Globulin Prolamin Aqueous Alcohol (Ethanol) Salt (NaCl) Solution Liquid Mixing Solid Centrifuge Globulin Isolation Liquid Solid Mixing Centrifuge Prolamin Isolation Liquid Solid Alkali solution (NaOH) GlutelinCentrifuge Glutelin Isolation Liquid Solid Substrates for Fiber Extraction

Protein Extraction : Scheme II Protein solubilization Defatted BB Centrifuge Solid Substrates for Fiber Extraction Liquid Protein Isolation Centrifuge Protein Ethanol NaOH pH control

Fiber Separation Substrate from protein extraction Treatment (Acid or alkali) Centrifuge Fiber Isolation (Precipitation) Centrifuge Ethanol Soluble Fiber Insoluble Fiber for Pretreatment Liquid Solid Liquid

Pretreatment & Lignin Separation Substrates (Insoluble Fiber) Dilute-Acid Pretreatment Pure cellulosic material for enzymatic hydrolysis Solid Acid pH control Lignin Isolation Centrifuge Lignin Liquid Centrifuge Solid