Single cell assay on CD-like lab chip using centrifugal single cell trap Microsystem Research Center, Korea Institute of Science and Technology 報告人：微機電所 陳治忠

Types of signaling

Conventional method to isolate single cell

Chip design consisting of chamber channel and valve, and trap

Experimental setup Control module CCDMotor parts of for rotating chip

Microfluidic component on CD-chip Photo image of microfluidic pattern Schematic sideview of CD-chip

Cell trap assay Cell trap VS duration timeCell trap VS trap size

Survival rate of cells with the treatment of PFA Single cell assay at CD-chipMTT assay of crowed cells at 96 well plates

Rate of apoptotic cells with the expose of UV Fluorescence microscope image of dead cells Fluorescence intensity data was cutoff at 10% of average value

Conclusion The ratio of trapping cells were almost 80% and more than 600 cells can be test in a experiment The trapping efficiency was main effected by the size of trapping hole Cytotoxicity assay showed the single cell might not be affected by the neighboring cells

Fluorescein isothiocyanate (FITC)