OEB 192 – 11.10.17 Dynamics of adaptation. (Miki Lee, Nigel Delaney, Maryska Kaczmarek, Lewis Ward) In humidified, 30 °C room: 48-well plates Plate-shaking.

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Presentation transcript:

OEB 192 – Dynamics of adaptation

(Miki Lee, Nigel Delaney, Maryska Kaczmarek, Lewis Ward) In humidified, 30 °C room: 48-well plates Plate-shaking tower (x38) De-lidding station Twister arm Plate reader Custom software (system, data) Growth analyzed via automated, robotic system (Delaney et al., in prep) OD 600 ln(OD 600 ) 6 replicates… t D = h 95% ci = ±0.006 h R 2 > 0.999

Fitness assayed via competitions (Lee et al., Evolution) EvolvedFluor. ancestor T0T0 CompetitionFlow Cytometry Competitive growth T1T1 T1T1 fluorescence EvolvedFluor. ancestor T0T0 fluorescence W = (ln(E 1 /E 0 )) / (ln(A 1 /A 0 )) (David Chou)

Rapid improvement in 600 generations (Chou et al., Science; Chubiz et al., In prep) 2. Mainly M-specific 3. Fitness ≈ growth rate 1. Fitness gains large but varied 8 populations & average Population 4 Isolate to sequence Population 4 Why such a range?

(Paquin & Adams, 1983)

Wednesday (10/19): Tradeoffs, specialization & pleiotropy