Instrument Performance --Tests Field illumination (plastic slides, beads) Axial resolution (mirror) Chromatic aberration (1 u beads, mirror) Laser power.

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Presentation transcript:

Instrument Performance --Tests Field illumination (plastic slides, beads) Axial resolution (mirror) Chromatic aberration (1 u beads, mirror) Laser power (meter, test sample) Laser stability (plastic slide, transmission detector) Beam alignment (focal check,mirror,bead)

Deviation + 8%

Deviation + 4%

Deviation + 17%

Deviation = +10%, +17%, +13%

UV Field Illumination 100X Objective (1.4 NA) 12.5% intervals INTENSITY=

5X Objective (.25 NA) 12.5% intervals

20X Objective (.7 NA) 12.5% intervals

Axial Registration Mirror Test 63X objective (1.2NA) (Chromatic Aberration)

63x ( 1.2NA) objective

Bad Galvanometer

568 nm 488 nm + 568nm 568 nm

Test Samples Beads: Spherotech 10 micron, MP focal check, 1 micron, submicron Front silvered mirror Silicon chip Biological samples-Diatoms, Pollen, MP Fluo slide, User defined specimen Fluorescence Plastic Slide

ABC D E F C

Lower NA’s --Less resolution Longer working distance Cleaner working environment Dry Objectives

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