Molecular Microbiology

Genetic Recombination Vertical –Binary fission –Budding Horizontal –Conjugation –Transduction –Transformation

Conjugation

Transduction

Transformation

Griffith Experiment

Biotechnology Recombinant DNA Genetic Engineering Disease treatments –SCID –SSA –CF Make products –Vaccines –Enzymes –Insulin –Hormones

Plasmids DNA Replicate independently Genetic Vectors –Small –Stable –Marker inserted –Gene expression

Background Donor Recipient Competent Cell –Natural Change cell wall Change PM –Artificial Temperature Salts (CaCl2) Marker Cloning Site

LB Broth and Agar Lysogeny Broth –Luria Broth –Luria-Bertani Nutritionally Rich –Peptides, casein –Vitamins –Minerals (NaCl) –Trace elements Biotech –Cultivate E.coli

Procedure Follow lab manual!! Reader –Directs –Keeps time Materials –LB plates 2 types –LB –LB-amp 2 situations –Before –After Label, Label, Label –pGreen plasmid –CaCl2 –Micropipets –Spreader –Loop inoculation Temperature –Incubator –Baths Ice Heat

Results Marker –Fluorescence Cloning site –R-ampicillin Colony observation –Natural light –UV light Calculate transformation efficiency

Questions? Glow Fish