Giansimone Perrino Tutor: Diego di Bernardo – co-Tutor: Mario di Bernardo XXIX Cycle - I year presentation Identification and Control of Gene Regulatory.

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Presentation transcript:

Giansimone Perrino Tutor: Diego di Bernardo – co-Tutor: Mario di Bernardo XXIX Cycle - I year presentation Identification and Control of Gene Regulatory Networks

Background Giansimone Perrino 2  Bachelor of Science in Computer Engineering – Università degli Studi di Napoli Federico II  Master of Science in Automation Engineering – Università degli Studi di Napoli Federico II  PhD Student in “Systems and Synthetic Biology Lab” of Diego di Bernardo at Telethon Institute of Genetics and Medicine (TIGEM) Fellowship provided by TIGEM

Problem Statement Giansimone Perrino 3 Idea: to use Control Engineering to regulate gene expression in eukaryotic cells

GAL1 promoter in Saccharomyces Cerevisiae Giansimone Perrino 4

Controller Results Giansimone Perrino 5

Products Giansimone Perrino 6 One paper in submission: i.A comparative analysis of strategies for in-vivo real-time control of protein expression from endogenous and synthetic gene networks Gianfranco Fiore, Giansimone Perrino, Mario di Bernardo, Diego di Bernardo ACS Synthetic Biology In Preparation

Next years Giansimone Perrino 7  Stochastic modeling and control of biological processes

… Giansimone Perrino 8

GAL1p control: in-silico experiments Giansimone Perrino 9

GAL1p control: in-vivo experiments Giansimone Perrino 10

Why? Giansimone Perrino 11 To be able to quantitavely assess the behaviour of living systems using an engineering approach. I.e. which concentration of a-synuclein is needed for aggregate formation in vivo? Right now in molecular biology, genes can only be induced to be ON or OFF but no fine regulation is possible.