Genomics for Triticeae improvement FP7 European Project.

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Presentation transcript:

Genomics for Triticeae improvement FP7 European Project

Overview Achieve significant progress in Triticeae genomics and support efficient breeding of improved varieties for European agriculture European Seventh Framework Programme project (financed by the EC) Project duration: 4 years – From June 2008 to Mai 2012 Budget: 7,5 million Euro (5,3 million Euro supported by the EC) Project Coordinator: Catherine Feuillet (INRA) Activities: 5 research WPs, 1 WP for project dissemination and 1 WP for project management

Overview 17 partners (including 2 breeders) from 9 countries Public Private

Activities and WPs

WP1: Physical mapping Of wheat group 1 and 3 chromosomes Construction of BAC libraries Fingerprinting of BAC libraries Assembly of BAC contigs BAC libraries pooling from the MTP and rearraying for BES (by INRA-CNRGV) BAC end sequencing of the MTPs for marker development and genome analysis

WP1: Physical mapping Assembly of BAC contigs Chromosome 3B: assembly by INRA (France) Chromosome 3D: assembly of 3DS by IEB (Czech Republic), and assembly of 3DL by JIC (UK) Chromosome 1B: assembly of 1BS by HU (Israel), and assembly of 1BL by INRA (France) Chromosome 1A: assembly of 1AS by UZH (Switzerland), and assembly of 1AL by SU (Turkey)

WP2: Physical maps anchoring Of wheat and barley group 1 and 3 chromosomes Establishment of a marker repository: including only markers for which sequences are available Generating resources for high resolution genetic mapping: For wheat, creation of a population of CS x Re RILs of about 2000 plants. For barley, creation of Morex x Barke RILs. Anchoring BAC contigs to chromosomes by high throughput screening methods: forward and reverse anchoring Comparative genetic and physical mapping with other grass genomes

WP3: Gene isolation and new alleles discovery Isolate five agronomically important genes and QTLs (4 in wheat and 1 in barley) TraitSpeciesChr.Partner Stripe rust resistance YrH52Wild emmer wheat1BSHU Wheat quality (pentosan viscosity) PV-QTL Bread wheat1BLINRA Glume blotch resistance QSng.sfr–3BS Bread wheat3BSUZH Grain yield QYld.idw-3BS Durum wheat3BSUNIBO Plant architecture (tillering) cul4 Barley3HUMIL

WP3: Gene isolation and new alleles discovery High-resolution mapping of the genes underlying the target traits Identification of candidate genes Functional validation of candidate genes New alleles discovery

WP4: Molecular markers for breeding WP leading by the breeding companies Development of trait specific markers: using markers from WP1 (BAC end sequencing) and WP2 Establishment of an European diversity panel for association mapping: panel contains 730 varieties (171 from UK, 178 from Germany and 381 from France) The data generated will be useful for all the chromosomes.

WP5: Bioinformatics tools and databases For wheat and barley Development of a web interface integrating genetics and physical maps Development of a new analytical framework for contig assembly: LTC (more robust than FPC) Development of comparative genome informatics tools: with Brachypodium, rice, sorghum, etc… Development of a new version of TriAnnot pipeline for automated sequence annotation

WP6: Dissemination, technology transfer and training Organisation of 9 training courses in the different partners instituts Development of the Project website Dissemination at scientific and technological events: talks/posters, production of a project leaflet and a poster

WP7: Project Management Scientific and technical management Administrative and financial management: organization of the different project meetings, monitoring of the project costs Communication and interactions between partners and other projects: establishment of a Collaborative Platform, interaction with the Triticeae Genomics Network (TGN) Technical and financial reporting to the EC : detailed reports that describe the work progress, costs incurred and other justifications for the reimbursement of project costs

UC Davis Kansas SU CSIC Viterbo U Modena U CRA-CER Foggia Murdoch U CSIRO UC Riverside Iowa SU Okayama U Minnesota SU CAAS Beijing CIMMYT MPI Cologne ACPFG A large network of international collaborators INTA UMB