Innovative integrated methodology for the use of decontaminated river sediments in plant nursing and road building CleAnsed: LIFE12 ENV/IT/ Expected start date: 01/10/2013Expected end date: 31/03/2016 Beneficiaries: CoordinatorISECNR: Institute for Ecosystem Studies of the National Research Council, Pisa, Italy Partners-CEBAS-CSIC: Consejo Superior De Investigaciones Científicas–Centro de Edafología y Biología Aplicada del Segura, Murcia, Spain -DICI: Department of Civil Engineering, University of Pisa -IBIMET: Istitute of Biometeorology of the National Research Council, Firenze, Italy -NAVI: Navicelli S.p.a. Pisa -UNIFI: Department of Plant, Soil and Environmental Sciences, University of Firenze, Italy Total budget1,655,370 € EU contribution827,683 € 2° Coordination meeting Pistoia-Italy 26 November 2014

CLEANSED: LIFE12 ENV/IT/ B. Implementation actions B1 Dredging, analysis and characterization of contaminated sediments D. Communication and dissemination actions D1 Project website D2 LIFE+ information boards D5 Press and media releases D6 Networking D10 Dissemination to Institutions and policy makers D13 After-LIFE Communication Plan E. Project management and monitoring of the project progress E1 Project management E2 Monitoring E3 Audit ISECNR Beneficiary responsible

B1 Dredging, analysis and characterization of contaminated sediments Analysis of the sediments dredged from the Navicelli canal from a physical, chemical, biochemical and toxicological point of view (ISECNR, CEBAS, UNIFI and DICI) -Chemical analyses: pH, Electrical Conductivity, Total and Available nutrients, Cation Exchange Capacity, humic matter - Biochemical analyses: hydrolytic and oxidoreductase enzyme activities -Toxicological analyses: Total heavy metals and available heavy metals (Fractionation), PAH, other toxic compounds, Germination index -Plant analyses: Growth and yield parameters, heavy metals -Physiscal analysis: Texture, Bulk density, Particle density, Water Retention Curve -Mechanical analyses Choice of the suitable treatment/post-treatment for sediment reclamation (ISECNR and DICI) CLEANSED: LIFE12 ENV/IT/ ISECNR Beneficiary responsible

CLEANSED: LIFE12 ENV/IT/ B. Implementation actions B2 Sediment treatment (Landfarming) B3 Demonstration of the use of decontaminated sediments as a substrate for plant nursing - Setup of demonstration sites (ISECNR, IBIMET) C. Monitoring of the impact of the project actions C1 Monitoring and validation of treated sediments -analysis and characterization of the treated sediments from a chemical, biochemical and toxicological point of view in order to evaluate if the reclamation has been successful ( ISECNR, CEBAS). D. Communication and dissemination actions D.9 Demonstration workshop, seminars, conferences and other events ISECNR Participation

ISECNR Monitoring Parameters Physical-chemicalPhysical-chemical ( e.g., Texture, Total and Available nutrients, humic matter etc.) BiochemicalBiochemical: -Enzyme activities (  -glucosidase, phosphatase, protease, dehydrogenase) - Humic-bound enzymes Very sensitive (dynamic conditions) Soils remain lifeless without enzymes. BIOINDICATORS AT ECOSYSTEM LEVEL change slowly (static conditions) B Actions CLEANSED: LIFE12 ENV/IT/000652

METHODOLOGY Post- Treatment Landfarming Sediment treated with AGRIPORT technology Fresh dredged Sediment Road construction Treatment Landfarming H2O, Organic H2O, Organic matter and organic contaminant eduction reduction Nurseries CLEANSED: LIFE12 ENV/IT/000652

AEREATIONNUTRIENTS Contaminated sediment Clay HDPE Sand Perforated pipe LANDFARMING sprinkler Landfarming is a bioremediation technology in which contaminated sediment is spread on the upper soil zone or in biotreatment cells, mixed with nutrients and the material is periodically irrigated and aerated. Organic contaminants are degraded by microorganisms and used as carbon source for growth and energy. B2 Sediment treatment Landfarming After the landfarming treatment the sediment will be used in environmental application as material for road construction (DICI) CLEANSED: LIFE12 ENV/IT/000652

Fresh dredged Sediment: Landfarming Clear decrease in C and N with the landfarming process due to the activation of the sediment microbial biomass, as confirmed by the increase in the dehydrogenase activity and of all the other hydrolytic enzyme activities T1 after 1 month and half T2 after 4months CLEANSED: LIFE12 ENV/IT/000652

Fresh dredged Sediment: Landfarming microbial stimulation caused a reduction in the organic contaminant concentration (about 20%) CLEANSED: LIFE12 ENV/IT/000652

Sediment treated with AGRIPORT technology: post treatment Landfarming PV: Paspalum vaginatum C: Controllo(no piante) TP: Tamarix+Paspalum SP: Spartium+Paspalum P: Phragmites OP: Oleander +Paspalum microbial stimulation caused a further, even if small, significant reduction in the organic contaminant concentration (15-20%) T1 after 1 month T2 after 2 months CLEANSED: LIFE12 ENV/IT/000652

T1 after 1 month T2 after 2 months the microbial activities resulted already stimulated with the landfarming treatment PV: Paspalum vaginatum C: Controllo(no piante) TP: Tamarix+Paspalum SP: Spartium+Paspalum P: Phragmites OP: Oleander +Paspalum Sediment treated with AGRIPORT technology: post treatment Landfarming

Action B3. Demonstration of the use of decontaminated sediments as a substrate for plant nursery CLEANSED: LIFE12 ENV/IT/ Sediment treated with AGRIPORT technology Plants: 3 ornamental species: Viburnum tinus L. (1), Eleagnus macrophylla L. (2), Photinia x fraseri var. Red Robin (3) Treatments: 3 mixtues of sediment and soil : 50:50 – 33:66 – 0:100 For each treatment experimentation carried out in two replicates Centro Sperimentale per il Vivaismo (Ce.Spe.Vi, Pistoia) 1 2 3

Parameters analysed: pH, E.C., Total Organic Carbon, Total Nitrogen, Nitrate, Ammonia, Available phosphorus, Dehydrogenase activity,  -glucosidase activity, Phosphatase activity Parameters under analyses: Humic Carbon Water Soluble Carbon Protease activity Butirrate reductase activity Action B3. Demonstration of the use of decontaminated sediments as a substrate for plant nursery Centro Sperimentale per il Vivaismo (Ce.Spe.Vi, Pistoia) Samples from the two replicate basins have been collected and analyzed immediatly after soil-sediment mixture preparation and after six months from plantation (0-15 and cm detpths); the results are the average of the two replicates Sediment treated with AGRIPORT technology CLEANSED: LIFE12 ENV/IT/000652

Sed LF: sediement at the end of landfarming ctl:control 100% agronomic soil T33: sediment:soil : 33:66 T50: sediment:soil : 50:50 Sediment treated with AGRIPORT technology: plant nursery at the initial sampling time (T0)

pH Electrical Conductivity Sediment treated with AGRIPORT technology: plant nursery after six months from plantation

Total Organic Carbon Total Nitrogen Sediment treated with AGRIPORT technology: plant nursery after six months from plantation

Nitrate Ammonia Sediment treated with AGRIPORT technology: plant nursery after six months from plantation

Available Phosphorus Sediment treated with AGRIPORT technology: plant nursery after six months from plantation

Dehydrogenase activity Sediment treated with AGRIPORT technology: plant nursery after six months from plantation

 -glucosidase activity Phosphatase activity Sediment treated with AGRIPORT technology: plant nursery after six months from plantation sed

Technical activity until now  Landfarming of Agriport treated sediments (2 months)  Application of sediments in nursery (ongoing)  Landfarming process of fresh dredged sediments (about 5 months)  Application of sediments in road construction (ongoing) Future technical activity  Construction of 100m road  Monitoring of plants, permeability and water infiltration rate in nursery activity ( IBIMET)  Analysis of sediment in nursery activity (CEBAS, ISE, UNIFI, Eontych)  31 March 2015 mid term CLEANSED: LIFE12 ENV/IT/000652

 Video and photos of technical and dissemination activities  Sent to the coordinator a little resume (3-5 lines) of the technical and dissemination activities by the end of each month (about 25)  Define further sampling times and parameters  Every three months send all financial document to the coordinator Very important issues GOOD WORK CLEANSED: LIFE12 ENV/IT/000652