H1299 Vector H1648 TUSC2 TUSC2 -ER 2.3 uM ER H1299 Vector TUSC2 TUSC2 -ER 2.3 uM ER H1299 Vector H1648 TUSC2 TUSC2 -ER 2.3 uM ER H1299 Vector TUSC2 TUSC2.

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H1299 Vector H1648 TUSC2 TUSC2 -ER 2.3 uM ER H1299 Vector TUSC2 TUSC2 -ER 2.3 uM ER H1299 Vector H1648 TUSC2 TUSC2 -ER 2.3 uM ER H1299 Vector TUSC2 TUSC2 -ER 2.3 uM ER FGFR2 TUSC2 Actin FGFR2 48 hours 24 hours Positive TUSC2 repressed FGFR2 expression in H1299 cells.

TUSC2 repressed FGFR2 expression in H1650 cells but not H322 cells. FGFR2 H1650 H322 FGFR2 FGFR2 expression is very low in H322 cells Vector TUSC2 TUSC2 -ER 2.3 uM ER H hours Vector TUSC2 TUSC2 -ER 2.3 uM ER H1650 H322 Vector TUSC2 TUSC2 -ER 2.3 uM ER H1650

Repeat western blotting in A549 cells FGFR2 TUSC2 Vector TUSC2 TUSC2 -ER 2.3 uM ER A549 Vector TUSC2 TUSC2 -ER 2.3 uM ER A549 Vector TUSC2 TUSC2 -ER 2.3 uM ER FGFR2 A hours 48 hours 24 hours There are two bands near the size of 120kd. TUSC2 seems repressed the upper band at 24 hours time point.