Genome Editing Dieter Steinmetz ZMBP Universität Tübingen Kurs SS 2015

„Übersichtsfolie“ ZFN „Zinc Finger Nuclease“ TALEN “Transcription Activator-Like Effector Nuclease” CRISPR/Cas9 nuclease “Clustered Regularly Interspaced Short Palindromic Repeats/Cas9 nuclease” ZMBP Quelle: 1. Baltes & Voitas und 3. Wikimedia Commons

„Kapitelfolie“ ZFN – „Zinc Finger Nuclease“ TALEN – “Transcription Activator-Like Effector Nuclease” CRISPR/Cas9 nuclease – “Clustered Regularly Interspaced Short Palindromic Repeats/Cas9 nuclease” ZMBP

ZFN – TALEN – CRISP – gleicher Mechanismus Suchsequenz DSB Disruption oder Substitution ZMBP Chen et al. GigaScience :24 doi: / X-3-24

„Ergebnisfolie“ Fig A Fig B Fig C Fig D ZMBP Merkle FT et al. (2015). Efficient CRISPR-Cas9-Mediated Generation of Knockin Human Pluripotent Stem Cells Lacking Undesired Mutations at the Targeted Locus. Cell Reports. Volume 11, 6, 12 May 2015, Pages 875–883