01/08/2015 Biotech-based world leader in enzymes

NOVOZYMES PRESENTATION01/08/20152 Biotech-based world leader in enzymes From agricultural products to enzymes Large Scale Manufacture of Enzyme products

NOVOZYMES PRESENTATION01/08/20153 Production of enzymes Fermentation Fermentation of a selected microorganism (bacterium or fungus) under sterile conditions Production of the desired enzyme by consumption of carbohydrates, proteins, salts, water and energy Transfer of the fermented broth to Recovery Fermenter Recovery; filtration, purification, stabilization etc. Granulation; mixing, drying etc. Finished products; in drums or big bags Recovery Filtration of the fermentation broth Purification of the liquid phase containing the enzyme Concentration of the liquid enzyme to desired enzymatic activity Stabilization & standardization of a liquid product – or Standardization of a liquid concentrate for granulation Granulation Formulation and standardization of the enzyme into solid granules to encapsulate the enzyme - and provide the right property of the product (solubility, colour, etc.)

NOVOZYMES PRESENTATION01/08/20154 How to make enzymes 4 Main Process Steps  Fermentation  Recovery  Granulation  Environmental Operations

NOVOZYMES PRESENTATION01/08/20155 Fermentation Compressor Mixing of nutrient medium Water Inoculation flask Seed fermenter Air Sterile filter Fermented broth for recovery of enzyme Cooling water Exhaust Sterilization Raw materials Carbohydrates: Ground grain/corn Starch Glucose Sugar Proteins: Soy Beans meal Gluten Corn steep liquor Casein Salts: Phosphates Sulphates Ammonium salts From basic raw materials + “the right micro organism” to a “soup” (broth) with enzymes and bio-mass

NOVOZYMES PRESENTATION01/08/20156 Enzyme recovery Fermenter Cooling Ultrafiltration Stabilization Bacterial filtration Bacterial filtration ABAB Drum filtration Filtration of enzyme crystals Pretreatment Filter aid is added Preservatives are added Crystallisation Culture broth Enzyme crystals for granulation Liquid concentrate for granulation Liquid product From a broth with enzymes to a purified solution of enzymes (or a liquid product)

NOVOZYMES PRESENTATION01/08/20157 Granulation Raw materials Granulation mixer Fluid-bed dryer Sifter Hopper Fibre drums Coating mixer Fluid-bed cooler Processing aids are added Big Bag From a purified solution of enzymes to a tough granulate Enzyme crystals or liquid concentrate from the recovery plant Granulated final product

NOVOZYMES PRESENTATION01/08/20158 Optimisation of the biological production Micro organisms Energy Electricity Steam Water Enzymes NovoGro Process air - fermentation Wastewater Raw materials Carbohydrates Proteins Glucose Starch Soy grits Other proteins Vitamins Salts Process

NOVOZYMES PRESENTATION01/08/20159 Quality control Control Points  Analyses  Process inspection Proces in Control  Control cards  Minimise variation Quality Reviews  Customer complaints  Process deviations  Off spec Focus on prevention

NOVOZYMES PRESENTATION01/08/ Environmental operations Biological wastewater plant  1,889,000 m 3 /year  1,040 tons COD (1) out ”Bio”-filtration of air Biomass converted to fertilizer ”NovoGro”  50,000 tons dry material per year  320,000 m3 total volume ISO certification in 2000 Most energy-efficient company award in November 2001 (1) Chemical Oxygen Demand