Nucleic Acid Purification

Stratagene’s Products for Nucleic Acid Purification

Strataprep Miniprep Kits n Kits for isolation of DNA from plasmid minipreps, PCR amplifications and agarose gels utilize a special silica-based fiber matrx to capture DNA in the presence of a chaotropic salt (guandinium) n High yields of extremely pure DNA n Fast, easy protocol- simply capture, wash and elute DNA n Convenient microspin format compatible with virtually all microfuges n No phenol, resin slurries or alcohol precipitation n DNA elutes in low ionic strength buffer or water and is application ready

Key Selling Points n Plasmid Miniprep Kit –Works on all sizes of DNA 2.9 kb plasmids to 45 kb cosmids –Up to 20 ug DNA from ml of culture –DNA transforms E. coli as easily as cesium purified DNA –Takes as little as 2.5 minutes/prep for 30 cultures at once n PCR Purification Kit –Up to 10 ug of PCR Product rapidly purified –Nearly quantitative removal of PCR primers and efficient removal of nonspecific amplification products smaller than 100 bp –Easy procedure does not require removal of oil overlay n DNA Gel Extraction Kit –Rapidly purify up to 10 ug DNA/prep –conventional and low melt agaroses and all neutral gel buffers –75-80% recovery of fragments 100 bp to 23 kb

Market Potential n The market potential worldwide for small- scale DNA purification is estimated at over $100 million –30% of the market is comprised of commercial kits and reagents –70% of the market relies on traditional methods and procedures (phenol extraction and ethanol precipitation)

Who is the Competition? n Qiagen and Promega have about 30% market share in the small scale purification market (1997) –Qiagen ca. $13 million –Promega ca. $8 million n Clonetech has recently entered the market promoting NucleoBond, NucleoSpin and NucleoTrap nucleic acid purification products n Competing claims about “miniprep speed”

Competitive Comparison n StrataPrep Plasmid Miniprep Kit was tested side-by-side with Qiagen and Promega and found to be as fast, and in some cases faster! n StrataPrep purified PCR products yield nearly TWICE the transformation efficiency as inserts prepared with Qiagens silica-gel membrane method n Side-by-side testing with Qiagens kit shows that StrataPrep PCR purification procedure is superior at removing contaminants n Compared to Qiagens Qiaex II method, DNA purified using StrataPrep DNA Gel Extraction Kit has higher cloning efficiencies

Product Positioning n StrataPrep Kits address our customers evolving DNA purification needs n High yields of DNA can be used immediately in todays demanding molecular studies –Cloning –Mutagenesis –Sequencing –Probe Generation n Convert customers by superior performance (yield, purity, speed) and value (economically priced and volume pricing for 250 count kits)

StrataPrep EF Plasmid Midiprep Kit n More than just a Midiprep n Yields up to 350 ug plasmid DNA n Fast and easy protocol n Yields endotoxin-free DNA (optional step) n No additional ethanol precipitations needed n DNA is ready for ALL applications including automated sequencing and mammalian transfections

StrataPrep EF Plasmid Midiprep Kit n Utilizes the same silica-based fiber matrix technology as other StrataPrep kits n Processing time is 90 minutes or less (includes endotoxin-free step) vs. 3+ hours n Unique color indication protocol to aid in successful purification n Few kits available with endotoxin-free purity

StrataPrep EF Plasmid Midiprep kit Competition n Qiagen gravity drip columns purchase endotoxin removal buffer separately n Promega not transfection grade drip columns n Clontech not really transfection grade

Who Are Our Customers? n Researchers utilizing small scale DNA purification technologies can be found across the life science spectrum –Academic, Government and Private Research Labs –Diagnostic Testing Labs –Biotech –Pharmaceutical

Purification Product Sales Tools n Catalog n Product Profiles n Strategies Articles

The StrataPrep family grows... Not only do we have DNA purification...

StrataPrep Total RNA Miniprep Kit n High yields of quality Total RNA n Fast and easy protocol n Purifies total RNA from cells and tissue n Microspin cup configuration (same StrataPrep microspin cup chemistry) n DNase digestion performed on column (no phenol extraction or ethanol ppt) n RNA ready for all applications (Northerns, RT-PCR, RNase protection assays, etc.)

StrataPrep Total RNA Miniprep Kit Competition n Qiagen not DNA free, but good yields of RNA n Promega similar spincup config. but varying results (odd protocol) (also recommend as a DNA purification kit) n Boehringer Mannheim DNA free but only recommended for cells, not tissue

StrataPrep Total RNA Miniprep Kit Features and Benefits n High yields of quality Total RNA n Fast and easy protocol n Purifies total RNA from cells and tissue n Microspin cup configuration DNase digestion performed on column n RNA ready for all applications (Northerns, RT-PCR, RNase protection assays, etc.)

Genomic DNA DNA Extraction n Gentle digestion of cellular proteins followed by removal of proteins by “salting-out” n 100 to 350 kb range from blood n 100 kb from tissue n No Organics

Genomic DNA DNA MicroExtraction Kit u Samples as small as 1 ul of blood u Quick (1 hour) u No Organics u Very reliable u Strategies article Quick Isolation of DNA Suitable for PCR Using Minute Amounts of Whole Blood vol. 5 pg. 68

StrataClean Resin n Patented hydroxylated silica particles. Offers a rapid, nontoxic alternative to phenol/chloroform for small volume extractions. n Extracts unwanted enzymes from DNA RE, PCR, and sequencing template prep n Concentrates dilute proteins for micro- analysis

ClearCut™ Miniprep Kit n Simple 20-minute plasmid prep method (modif. alkaline lysis, silica resin) n No phenol/chlororform or cesium banding n Produces pure plasmid for: RE, ligation, seq, transformations n Buffer exchanges and PCR cleanup in 5 minutes

RecoverEase DNA Isolation n High-molecular weight genomic DNA from whole tissue n No organic extractions or ethanol precipitations n DNA is ready to use the next day n Ideal for use with the Big Blue system and transgenic mutation assays

Stratagene has the Purification Solution! n Stratagene has a method for all your customers nucleic acid purification needs n The new StrataPrep Kits quickly purify high quality DNA from minipreps, PCR amplifications and agarose gels n When only small amounts of material are available use the Micro DNA and RNA Kit formats