Blanche D’Anastasi – PhD Candidate 1,2,3 Dr Vimoksalehi Lukoschek – Research Fellow 1 Dr Lynne van Herwerden – Senior Lecturer 2 Dr. Colin Simpfendorfer – Professor and Centre Director 3,4 Dr. Jean-Paul Hobbs – Post Doctoral Fellow 5 1 Australian Research Council Centre of Excellence for Coral Reef Studies, JCU 2 School of Marine and Tropical Biology, James Cook University 3 School of Earth and Environmental Sciences, JCU 4 Centre for Sustainable Tropical Fisheries and Aquaculture, James Cook University 5 Oceans Institute, University of Western Australia Simon Allen

1.Extract DNA from intestinal content of at least 20 snakes per species 3.Pool equal amount of amplicons from 25 snakes

4.Send for Next Generation Sequencing on a Roche GS Junior 454 platform 5.A library of 7000 sequence reads per snake 6.Use Bioinformatics platforms to match each read to a data bases

7.No match – geographic information or assign higher taxonomic status 8.Calculate % contribution of each prey item Murray et al., 2011