From Natural Diversity to Better Cultivars 10 January 2013 San Diego, California

Evaluation of needs and priorities has been ongoing in the current RosBREED project: Information of trait values from stakeholders (Obj. 1 of RB1) Information of MAB breeding needs and opportunities from breeders (Obj. 5 of RB1) Input from Advisory Panel Members (Obj. 5 of RB1) Direction from Feedback

Feedback Received Industry: Risks and opportunities indicate a need to broaden the scope of traits while making sure that fruit quality is still a top priority Breeders: MAB adoption has begun, but breeders need help (access to routine accurate genotyping services, genetic knowledge for other traits, implications of trait locus clusters, and knowledge of genotype × environment interaction)

Planning Activities in 2012 Advisory Panel Members: recommendation to consider a second project U.S. Rosaceae Executive Committee: participation in conference calls and meetings U.S. Rosaceae breeders and interested colleagues: written input solicited, involved in planning process Planning group: developed Logic Model Planning group: identified scope, desired outcomes, and objectives Advisory Panel members: provide input on scope, desired outcomes, and objectives TODAY

More rapid availability of new cultivars with genetically superior fruit quality Improved profitability and sustainability of U.S. rosaceous fruit, nut, and floral crops with increased consumption and enjoyment RosBREED’s Long Term Outcomes

RosBREED Approach Improve the efficiency and success of U.S. Rosaceae crop breeding programs through the use of DNA diagnostic tests

RosBREED #2

IMPLEMENTATION DISCOVERY Pre- breeding Post- breeding Breeding Plant diversity identified to address risks & opportunities Risks mitigated, opportunities realized Wild germplasm Elite germplasm Cultivar release & adoption

Objectives Structure Obj. 1. Central breeding support Obj. 2. New trait locus discovery Obj. 3. Pre-breeding Obj. 4. Post-breeding Obj. 5. Communicate project outcomes & impacts

Which wild germplasm to use? Which parents & combinations to create? Which seedlings to progress? Which selections to trial? Which advanced selections to commercialize? Supporting Breeding Decisions

Obj. 1: Central Breeding Support Goal: Expand support tools Rosaceae-wide for efficient processing of new cultivar-generating plant populations addressing production and market risks and opportunities Success: Valuable & superior new genetic combinations efficiently, accurately, rapidly, and creatively developed and identified; Massive complex breeding data managed efficiently for routine implementation of DNA-informed breeding

RosBREED #2

Demonstration Breeding Programs

Opportunities Addressed Advances in comparative genomics will allow for more efficient genetic discovery across crops A new statistical approach (Genome-wide selection) has potential to increase the precision of marker-assisted breeding Advances by international groups/projects will be leveraged

Challenges Addressed Access to accurate, cost effective genotyping services Database resources and tools to support MAB Polyploidy: defining functional alleles & allele dosage

Obj. 2: New trait locus discovery Goal: Ensure known trait loci are available for high-priority traits addressing production and market risks and opportunities Success: All U.S. Rosaceae breeding programs have access to promising known trait loci addressing high value traits

Which wild germplasm to use? Which parents & combinations to create? Which seedlings to progress? Which selections to trial? Which advanced selections to commercialize? Supporting Pre-Breeding Decisions

Obj. 3: Pre-breeding Goal: Provide access to genepools of wild and novel germplasm for rosaceous crop breeders Success: Valuable alleles from wild or novel germplasm are routinely identified and incorporated into cultivar-generating populations more efficiently, accurately, rapidly and creatively

Which wild germplasm to use? Which parents & combinations to create? Which seedlings to progress? Which selections to trial? Which advanced selections to commercialize? Supporting Post-Breeding Decisions

Obj. 4: Post-breeding Goal: Enable and optimize use of DNA information for elite selection advancement and cultivar adoption decisions Success: Breeders and industry sectors will make confident elite selection advancement and cultivar adoption decisions, thereby mitigating risks and realizing opportunities

Obj. 5: Communicate project outcomes and impacts Goal: Communicated project outcomes and impacts to all stakeholders Success: Breeders, allied scientists, industry sectors, and the public have an increased understanding of how genetics knowledge is being used to more efficiently and rapidly develop and commercialized new rosaceous cultivars that provide increased consumption, enjoyment, profitability, and sustainability

U.S.-Wide and Multi-Disciplinary BREEDING GeneticsGenomicsBioinformatics Computer Programming Socio Economics EvaluationCommunication Technology Transfer

Plant Research International, Netherlands East Malling Research, UK CRA-FRU, Rome, Italy IASMA, Trento, Italy INRA ( Bordeaux, Avignon, Angers ), France Andres Bello University, Chile Plant & Food Research, New Zealand ARC, South Africa RosBREED participant location International Collaborators + 2 Scientific Advisory Panel members (Spain, New Zealand)

Acknowledgement Contributors to Logic Model Cameron PeaceAmy IezzoniRobert Shupp Nahla BassilSook Jung Eric van de Weg Rex BernardoJim LubyGayle Volk Michael CoeDorrie MainVance Whitaker Tom DavisChris MaliepaardChengyan Yue Kate EvansVicki McCracken Gennaro FazioJim McFerson Karina GallardoJay Norelli Ksenija GasicMercy Olmstead Craig HardnerBrad Rickard

Advisory Panel Members RosBREED I First Annual Meeting, 7-8 Jan 2010 RosBREED II Annual Advisory Panel Meeting, 13 Jan 2011

Thank you