Radiation Safety at Tufts Helpful Hints for Radioisotope Users.

Slides:



Advertisements
Similar presentations
Lab Safety.
Advertisements

Safety First Chemistry Fall 2011.
Chemistry safety Part 2.
It is better to be careful a hundred times than be killed once.
General Safety Rules Chemistry 11.
Radiological Emergency Procedures FOR ALL ISOTOPES 1.Prevent others from entering the affected area or coming into contact with the potentially radioactive.
Lab Safety. Know the Location of the Following… Eyewash Fire blanket Fire Extinguisher.
Chemistry Laboratory 1- General Safety Rules 2- Chemical Safety 3- Glassware and Equipment.
Lab Safety Guidelines. Hand Sanitation is Essential Wash your hands anytime you start cooking, change kitchen jobs, need to put on gloves, use the restroom,
Emergency Response to Biological Incidents Response to Biological Spills in the Laboratory (Intentional or Accidental)
Klebba Lab Rules and Regulations July Golden Rules If you open it, CLOSE IT If you turn it on, TURN IT OFF If you break it, REPORT THE BREAKAGE.
© 2006 Plano ISD, Plano, TX. Working with Heat Fire or.
Laboratory Safety.
Tulane University - Office of Environmental Health & Safety (OEHS) HAZARDOUS MATERIAL SPILLS For Tulane Laboratory and Facilities Employees May 2012.
Prepared by; Miss Syazwani Mahmad Puzi School of Bioprocess Engineering UniMAP.
PROCEDURES AND REQUIREMENTS FOR THE SAFE USE OF RADIOACTIVE MATERIAL Indiana University - Bloomington.
LABORATORY SAFETY RULES AND GUIDELINES FOR WORKING IN CHEMISTRY LABS AT OCCC.
Laboratory Safety Safety Rules Be prepared to work when you arrive at the laboratory. Familiarize yourself with the lab procedures before beginning.
Laboratory Safety Safety Rules Be prepared to work when you arrive at the laboratory. Familiarize yourself with the lab procedures before beginning.
Laboratory Safety. 1. Follow all instructions carefully.
Lab Safety Rules.
Chemistry Lab Safety Rules Bakersfield College Chemistry 2A Physical Science 12.
Lab Safety Rules Chemistry.
Physical Science1 Laboratory Procedures. 2Physical Science Fume Hood Located between lab and prep room Prevents harmful fumes from entering lab May be.
SAFETY IN CHEMISTRY LAB Everyone is Responsible! "I didn't mean to" and "It wasn't my fault" are two statements that have no place in the lab. Horse-play.
Safety in the Laboratory Kentlake Science Department.
SAFETY IN THE LABORATORY. Successful work in the science laboratory involves not only mastery of scientific concepts and techniques, but also knowing:
C HEMISTRY L AB S AFETY R ULES. S AFETY R ULES Wear appropriate protective clothing.
Safety is Your Responsibility
Lab Safety. Biological Science building room on map extra 5 min to get there.
Safety and Sanitation. When working with ELECTRIC APPLIANCES, the safety rules are: 2. Stand on a dry surface. 1. Keep your hands dry. 3. Keep electric.
FORENSIC SCIENCE LAB SAFETY.
Laboratory Safety Biology. Objective The student will demonstrate safe practices during field and laboratory investigation.
Safety and First Aid Know what to do in case of emergency.
Laboratory Safety Rules. 1. Be prepared to work when you arrive at the laboratory. Familiarize yourself with the lab procedures before beginning the lab.
7. EMERGENCY RESPONSE RYERSON UNIVERSITY.
Welcome to the wonderful world of Chemistry Miss Qumsieh.
LAB SAFETY Safety is Your Responsibility No Horse-play in the Lab……
Practical General chemistry Dr.Gharam Ibrahim. A -Lecturer Dr. Gharam Ibrahim; Office: third floor,chemistry department B -Instructors in the Laboratory:
CHEM 200 General Laboratory Safety. The word safety means the quality or condition of being safe; freedom from danger, injury, or damage. What is safety?
Lab Safety. When working in the Laboratory …. Tie back loose clothing Do Not wear open- toed shoes Wear goggles.
Chemistry TEK 1 Zack Ruiz Eve Villarreal. TEK 1) Scientific processes. The student, for at least 40% of instructional time, conducts field and laboratory.
Why even worry about all this lab safety stuff? Sodium Hydroxide to the skin Ammonia in the eye.
Safety Following are notes on general lab safety, proper dress for the lab and accident procedures.
Extremity Dose Reduction By Aggie Barlow, CHP, MS, MBA.
LABORATORY SAFETY MEDICAL MICROBIOLOGY. SAFETY RULES Wash your hands thoroughly with soap and water before leaving the laboratory and any time they become.
Safety is Your Responsibility
Lab Safety.
The safety contract must be signed by you (the student) and your parent or guardian, and returned before you can participate in activities. If you currently.
By following safety rules in the lab, you will help keep both yourself and others safe. Safety Rules.
SAFETY RULES. Safety Rules READ, READ, READ Locate –firealarms –eyewash Work with a buddy Declutter.
Please take out a piece of paper… What lab safety rules do you already know? (Must have at least 7 bullets)
Safety in Lab Environment. Safety Rules 1. Follow Instructions…Understand what you are going to do carefully before attempting to do anything. 2. Wear.
Laboratory safety rules Dalia Kamal Eldien Mohammed Practical NO (1)
Lab Safety Symbols Glassware safety Check the condition of glassware before and after using it. Inform your teacher of any broken, chipped, or.
TOPIC: Lab Safety AIM: How do we practice safety in the science laboratory? DO NOW: Why is it important to follow safety rules?
Lab Safety.
LABORATORY RULES You must "teach" lab safety !. LABORATORY RULES DEPARTMENT OF BIOLOGICAL SCIENCES 1. Act in a responsible manner, concentrate and work.
September 1, 2015 Section 1-1: Safety IN: What do you do in case of fire? What about broken glass? What if an unknown chemical spills on you or in your.
You will be doing many laboratory activities, which require the use of hazardous chemicals and expensive lab equipment. To ensure a safe science classroom,
Laboratory Safety General Rules The laboratory is for serious work. There will be no horseplay in the lab. Study your lab assignments before going into.
On science.
Laboratory Safety Rules.
SAFE LABORATORY PRACTICES
Lab Safety Unit 1 Lesson 2.
SAFE LABORATORY PRACTICES
Formalin Training Anatomy and Physiology Laboratory
Instructions for Wipe Testing for Radioactive Material Contamination
Safety and Sanitation.
Presentation transcript:

Radiation Safety at Tufts Helpful Hints for Radioisotope Users

Personal Protective Equipment Be sure your lab coat is long enough; close/button it completely whenever in the lab; do not roll up sleeves exposing bare skin. Lab coats gathered or with elastic at the wrist are preferred to ones that hang away from the wrist. Double gloving is recommended to prevent skin contamination if one glove has a hole or tears. Avoid open-toed shoes, shorts and skirts when working with radioactive materials.

Eye Protection and handling RAM Wear safety goggles anytime there is a splash hazard. This includes when pouring liquid radioactive waste, handling concentrated solutions, and vortexing samples. Know where the nearest eyewash is located and how to use it.

Using stock vials of radioactive material [RAM] Thaw completely before attempting to take an aliquot from a stock vial. Incomplete thawing may result in a frozen portion of concentrated radioisotope escaping from the vial. Such ice can slide across counters and floors leaving large areas contaminated.

Tube selection in the lab When using radioactive material, screw top tubes are better than flip top tubes. The more secure the closure of the tube, the less likely it is to pop open, ex. during centrifugation or when heating the contents. Securely closed tubes prevent spills.

Using incubators with RAM Whenever possible, place flasks containing RAM cultures or solutions in secondary containers to prevent spills and contamination of the incubator. Use the lowest shelf available in the incubator so that you are not reaching for containers that are over your head. Straining to reach a container can lead to a spill.

Incubators continued Open dishes or flasks containing radioisotope, especially some chemical forms of S-35 [ex. Methionine], can result in the water within the incubator becoming contaminated. Be careful opening the inner incubator door to avoid contact with condensation droplets. Place absorbent paper on the counter in front of the incubator to catch any contamination. Place the incubator as far back on the bench top as feasible. Be sure to survey in front of incubators to check for radioactive contamination.

Spin Columns and RAM Used spin columns can pose a radiation contamination hazard. When the powder matrix in the column dries, radioactively contaminated powder can be spread around the lab. Place all used spin columns in conical tubes or plastic bags, and then into the radioactive waste container to avoid contamination of the lab.

Ice buckets Ice buckets used to cool tubes of radioactive material frequently become contaminated. Dispose of the used ice in a sink designated for radioactive material disposal. Rinse the sink well to help the ice melt and to decontaminate the sink before anyone else uses it.

Centrifuge Balance the centrifuge well to avoid breakage and Contamination of the inside of the centrifuge. Wipe off tubes before centrifugation to avoid contaminating the inside of the centrifuge. Close tubes tightly, preferably using screw top tubes. Let the centrifuge stop completely before opening, esp. bench top models. Broken tubes in a moving centrifuge can spray radioactive material on personnel and the lab.

Shakers Place plastic-backed absorbent on a shaker to catch any spills. This allows one to dispose of the contaminated absorbent in the RA waste container, and can save hours of cleaning. This is especially important with shared equipment = no down time.

Gel Handling Wear gloves and lab coat when handling gels. Be aware of dose rate from P-32 containing gels. Dispose of as a solid or dry RA waste. Ensure gel does not run through processor with the autoradiography X-ray film.

Electrophoresis &Autoradiography Prevent fires with proper use of gel electrophoresis equipment. Ensure proper voltage and avoid running gels overnight when labs are not attended. Equipment should be labeled as containing RAM. Be sure autoradiography cassette is labeled as containing RAM so all potential handlers/users are aware. Cassettes must also be kept secure from loss or theft per MA RCP regulations.

Avoid contamination of a meter If your geiger counter probe is contaminated, you will not be able to perform safety surveys. Do NOT cover the probe with Parafilm, as this reduces detector efficiency, esp. with C-14 and S-35. Turn/hold the probe sideways to survey your hands so any droplets do not fall onto the pancake probe. Do not touch the probe to surfaces to survey – use ½ inch detector to surface distance.

Post Experimental Surveys Performing radiation contamination surveys periodically during RAM use and immediately after each use of RAM is the “heart” of a good radiation safety program. Find any contamination early, before it is spread around the lab or contaminates others. Frequent surveys are ALARA and save work in the long run.

Change of clothing It is recommended that those who work directly with radioisotopes keep a change of clothing for emergencies. Spills can happen. If one’s clothing is splashed with radioactive materials [or other chemicals] having a clean set of clothing readily available is very helpful. This can be your own clothing or a set of scrubs, which you can keep in a locker or desk drawer in case it is needed.