PROJECT LIFE12 ENV/IT/ “Anti-infective environmental friendly molecules against plant pathogenic bacteria for reducing Cu” “AFTER Cu” Start: 01/01/2014 End: 31/12/ , January 22nd I st year Meeting Faenza ASTRA premises

PROJECT LIFE12 ENV/IT/ AGENDA I st year meeting TECHNICAL & PROGRESS MEETING 15:00 [ASTRA] Welcome and opening of the meeting 15:05 [All] Actions carried out by each partner (please prepare a ppt presentation and include After-Cu logo and LIFE logo in each slide) and scheduled activities from June 2014 until the end of December :30 [All] Open discussion on actions carried out 17:00 [All] After-Cu Technical future issues: o [DISPAA] Technical activities scheduled in the next six months of the project o [All] Open discussion on actions to be carried out in the next six months of the project 17:30 [DISPAA] Management and dissemination issues 17:50 [All] General questions 18:00 End of progress meeting

PROJECT LIFE12 ENV/IT/ AFTER Cu – 1° year meeting Faenza – 2015, January 2015

PROJECT LIFE12 ENV/IT/ AFTER Cu – 1° year meeting Faenza – 2015, January 2015

PROJECT LIFE12 ENV/IT/ Technical Actions carried out by each partner in the last six months  B.2: Demonstration of the qualitative and quantitative yields of conventional chemical synthesis and biotechnological synthesis of the anti-virulence peptides at laboratory scale  B.3: Demonstration of the chemical and biological stability of anti-virulence peptides produced by conventional chemical and biotechnological synthesis  B.4: Demonstration of Kilo-scale biotechnological synthesis of the anti-virulence peptides by recombinant technology  B.5(close start): Demonstration of the null toxicity profile of the anti-virulence peptides on model organisms and microorganisms  B.6 (close start): Demonstration of the anti-virulence peptides in vivo performances at pilot scale level in field screenings  B.7 (close start): Demonstration of the effectiveness of the anti-virulence peptides in the presence of contamination by copper compounds at pilot scale level in field screenings. AFTER Cu – 1° year meeting Faenza – 2015, January 2015

PROJECT LIFE12 ENV/IT/ Technical Actions carried out by each partner in the last six months  C.2: Monitoring of the in vitro anti-virulence performances of peptides at laboratory level  C.3: Monitoring of the absence of side effects for the anti-virulence peptides on common targets of any living organism at laboratory level  C.4 (start): Monitoring of the absence of a direct selection operated by the anti- virulence peptides towards the emergence of bacteria resistant to the anti-virulence peptides themselves at laboratory level  C.5 (start): Monitoring of the short term environmental benefits from the use of the anti-virulence peptides in plant disease control at pilot scale level in field screenings  C.6 (start): Monitoring of the absence of an indirect selection operated by the anti- virulence peptides on copper compound and antibiotic resistant bacteria at pilot scale level in field screenings. AFTER Cu – 1° year meeting Faenza – 2015, January 2015

PROJECT LIFE12 ENV/IT/ Presentations of each beneficiary  CEBAS CSIS  ICCOM CNR  ASTRA  SOLDANO  DISPAA. AFTER Cu – 1° year meeting Faenza – 2015, January 2015

PROJECT LIFE12 ENV/IT/ Presentation of  DISPAA instruments and data referred to B2-B7 C2-C6 D actions. AFTER Cu – 1° year meeting Faenza – 2015, January 2015

PROJECT LIFE12 ENV/IT/ AFTER Cu – 1° year meeting Faenza – 2015, January 2015 Psn (0,5) + Agro_Ap17 C-

PROJECT LIFE12 ENV/IT/ AFTER Cu – 1° year meeting Faenza – 2015, January 2015 Psn (0,05) + Agro_Li27 C-

PROJECT LIFE12 ENV/IT/ AFTER Cu – 1° year meeting Faenza – 2015, January 2015

PROJECT LIFE12 ENV/IT/ AFTER Cu – 1° year meeting Faenza – 2015, January 2015

PROJECT LIFE12 ENV/IT/ AFTER Cu – 1° year meeting Faenza – 2015, January 2015

PROJECT LIFE12 ENV/IT/ AFTER Cu – 1° year meeting Faenza – 2015, January 2015

PROJECT LIFE12 ENV/IT/ AFTER Cu – 1° year meeting Faenza – 2015, January 2015

PROJECT LIFE12 ENV/IT/ AFTER Cu – 1° year meeting Faenza – 2015, January 2015 ….evaluated by Congo Red bioassay

PROJECT LIFE12 ENV/IT/ AFTER Cu – 1° year meeting Faenza – 2015, January 2015

PROJECT LIFE12 ENV/IT/ AFTER Cu – 1° year meeting Faenza – 2015, January 2015

PROJECT LIFE12 ENV/IT/ AFTER Cu – 1° year meeting Faenza – 2015, January 2015

PROJECT LIFE12 ENV/IT/ AFTER Cu – 1° year meeting Faenza – 2015, January 2015

PROJECT LIFE12 ENV/IT/ AFTER Cu – 1° year meeting Faenza – 2015, January 2015

PROJECT LIFE12 ENV/IT/ AFTER Cu – 1° year meeting Faenza – 2015, January 2015

PROJECT LIFE12 ENV/IT/ AFTER Cu – 1° year meeting Faenza – 2015, January 2015

PROJECT LIFE12 ENV/IT/ SAME STRATEGY APPLIED FOR AP17 AND Other PEPTIDES WORK In progress AFTER Cu – 1° year meeting Faenza – 2015, January 2015

PROJECT LIFE12 ENV/IT/ [All] Open discussion on actions carried out. AFTER Cu – 1° year meeting Faenza – 2015, January 2015

PROJECT LIFE12 ENV/IT/ AFTER Cu – 1° year meeting Faenza – 2015, January 2015

PROJECT LIFE12 ENV/IT/ Dissemination actions D1 Demonstration workshop in Italy (last 6 months) D2 Demonstration workshop in Spain (last 6 months) D3 Project website (end M3) D4 Notice boards (end M3) D5 Layman's report (last 3 months) D6 Diffusion material preparation D7 Articles and press releases D8 Networking D9 After Cu technology manual (last 9 months) D10 Demonstration workshop, seminars, conferences and other events D11 Dissemination to Institutions and policy makers D12 After-LIFE Communication Plan (last 3 months) D13 International fairs and other events D14 Digital supports for international diffusion AFTER Cu – 1° year meeting Faenza – 2015, January 2015

PROJECT LIFE12 ENV/IT/ Dissemination actions carried out by each partner in the last six months AFTER Cu – 1° year meeting Faenza – 2015, January 2015

PROJECT LIFE12 ENV/IT/ AFTER Cu Technical issues for the next six months… towards project conclusion..  B.2: Demonstration of the qualitative and quantitative yields of conventional chemical synthesis and biotechnological synthesis of the anti- virulence peptides at laboratory scale  B.3: Demonstration of the chemical and biological stability of anti-virulence peptides produced by conventional chemical and biotechnological synthesis  B.4 : Demonstration of Kilo-scale biotechnological synthesis of the anti- virulence peptides by recombinant technology  B.5: Demonstration of the null toxicity profile of the anti-virulence peptides on model organisms and microorganisms  B.6 : Demonstration of the anti-virulence peptides in vivo performances at pilot scale level in field screenings  B.7: Demonstration of the effectiveness of the anti-virulence peptides in the presence of contamination by copper compounds at pilot scale level in field screenings AFTER Cu – 1° year meeting Faenza – 2015, January 2015

PROJECT LIFE12 ENV/IT/ AFTER Cu Technical issues for the next six months towards project conclusion..  C.2: Monitoring of the in vitro anti-virulence performances of peptides at laboratory level  C.3: Monitoring of the absence of side effects for the anti-virulence peptides on common targets of any living organism at laboratory level  C.4: Monitoring of the absence of a direct selection operated by the anti- virulence peptides towards the emergence of bacteria resistant to the anti- virulence peptides themselves at laboratory level  C.5: Monitoring of the short term environmental benefits from the use of the anti-virulence peptides in plant disease control at pilot scale level in field screenings  C.6 : Monitoring of the absence of an indirect selection operated by the anti- virulence peptides on copper compound and antibiotic resistant bacteria at pilot scale level in field screenings  C.7 (start ) Monitoring of technical-socio-economic assessment of the After- Cu project AFTER Cu – 1° year meeting Faenza – 2015, January 2015

PROJECT LIFE12 ENV/IT/ After-Cu Technical future issues for the next six months and Open discussion on actions to be carried out in the next six months of AFTER Cu project AFTER Cu – 1° year meeting Faenza – 2015, January 2015

PROJECT LIFE12 ENV/IT/ AFTER Cu Dissemination issues for the next six months Discussion and Planning AFTER Cu – 1° year meeting Faenza – 2015, January 2015

PROJECT LIFE12 ENV/IT/ ADMINISTRATIVE ISSUES  Next Deliverables  Partner costs January 2015  Next meeting AFTER Cu – 1° year meeting Faenza – 2015, January 2015

PROJECT LIFE12 ENV/IT/ Partner costs January 2015 AFTER Cu – 1° year meeting Faenza – 2015, January 2015

PROJECT LIFE12 ENV/IT/ Courtesy of E.Colombi, 2012 AFTER Cu – 12 month meeting ASTRA – 2015, January 22nd Happy 1° birthday to AFTER Cu!

PROJECT LIFE12 ENV/IT/ AFTER Cu – 1° year meeting Faenza – 2015, January 2015

PROJECT LIFE12 ENV/IT/ AFTER Cu – 1° year meeting Faenza – 2015, January 2015

PROJECT LIFE12 ENV/IT/ AFTER Cu – 1° year meeting Faenza – 2015, January 2015

PROJECT LIFE12 ENV/IT/ AFTER Cu – 1° year meeting Faenza – 2015, January 2015