POROS® Affinity Columns Protein A, Protein G, and CaptureSelect® Affinity Columns Intended for analytical scientists supporting the development of biologic drugs. Or for small-scale purification of immunoglobulins, Fabs, or fusion proteins. POROS® Affinity Columns enable: Direct sampling from cell culture or fermentation harvests Welcome to this short presentation that is intended to provide a brief overview of the Poros Affinity Column range These prepacked Columns are intended for analytical scientists supporting the development of biologic drugs that require an assay to quantitate proteins produced in cell culture or fermentation harvests. They are also suitable for small scale purification of Immunoglobulins, Fabs, or Fusion proteins. POROS® CaptureSelect® HPLC columns incorporate the advantages of high-speed and high performance POROS® chromatography resins with the unique selectivity of Protein A, G and CaptureSelect ligands. The combination allows precise, three minute quantitation. In Addition the eluted product is highly purified and suitable for further analysis including mass Spectrometry Precise quantitation of product titers, typically in under 3 minutes with the eluted product being highly purified and suitable for further analysis including mass spectrometry.

POROS® Affinity Columns Prepacked column overview Product Name Specificity Dynamic Binding Capacity (mg/mL) POROS® A 20 Human, mouse, and 5 other species 19.0 POROS® G 20 Human, mouse, rat, and 8 other species 9.0 POROS® CaptureSelect® IgG Fc Human 8.9 POROS® CaptureSelect® IgM Human, mouse, and rat 4 POROS® CaptureSelect® LC Kappa Human, all subclasses 14.5 POROS® CaptureSelect® LC Lambda 10 POROS® CaptureSelect® HSA 2.6 POROS® prepacked columns Column Dimension Diameter (mm) x Length (mm) Column Volume (mL) 2.1 x 30 0.1 4.6 x 50 0.8 4.6 x 100 1.7 10 x 100 7.9 The Poros Prepacked Affinity Column range now includes 7 ligands immobilized on our Poros 20 µm beads and are offered in the four prepacked column sizes shown in the table to the right In the table to the left, the specificities of Protein A and G, shown in the top rows, are well documented. The Poros CaptureSelect range is the result of a Life Technologies and BAC collaboration. Unique specificities include: Poros®CaptureSelect®IgG-Fc which allows for the detection of any human IgG subclass (even IgG3) in the presence of IgG from other sources like bovine. Poros®CaptureSelect®IgM can be used for the analysis of human, mouse and rat IgM Poros®CaptureSelect®LC-kappa and Poros®CaptureSelect®LC-lambda products allow for the analysis of human antibodies and antibody fragments containing either a kappa or a lambda light chain. Poros®CaptureSelect®HSA product is well suited for analysis of HSA-fusion proteins and is specific for human serum albumin allowing for use even in the presence of albumin from other species. POROS® CaptureSelect® Columns A collaboration between Life Technologies and BAC

POROS® Affinity Columns Protein A, Protein G, and CaptureSelect® specificity Kappa or Lambda IgG Fc CH3 CH1 CH2 VH CL VL Protein A or Protein G POROS® Protein A & Protein G resins POROS® CaptureSelect® ligands Bimolecular type Protein A Protein G IgG Fc Kappa Lambda HSA IgM IgG1, IgG2, IgG4  IgG3 IgG, CH2 domain deleted IgA, IgD, IgE Fab, Fab'2 Human serum albumin Albumin fusion protein IgG Fc fusion protein A comparison of Protein A, G and Capture Select binding specificities is shown in the table and is also included in the Poros Affinity Column Product Bulletin The Antibody cartoon shows the location of binding epitopes for Protein A, G and the CaptureSelect ligands. Note that in situations where modifications to an antibody’s Fc region impact Protein A or G binding, the CaptureSelect IgG Fc product may provide a solution for quatitation or purification. The CaptureSelect Kappa and Lambda ligands both bind to the constant region of their respective light chains and bind all subclasses. In comparison, Protein L binds the VL domain of the Kappa light chain and exhibits strong affinity for 4 of the 7 subclasses. POROS® Protein A, Protein G, and CaptureSelect® ligands target multiple IgG epitopes. The graphic shows the binding sites for affinity ligands Protein A and Protein G. Novel POROS® CaptureSelect® ligands provide alternative specificities that enable rapid binding for the quantitation or purification of engineered immunoglobulins.

POROS® Affinity Columns Additional information Product Bulletin: POROS® Protein A, Protein G, and CaptureSelect® Columns Application Note: POROS® A 20 Analytical HPLC Columns Operating Instruction: POROS® Protein A, Protein G and CaptureSelect® Affinity Columns Additional information on performance and product specifications may be found in the product bulletin. The Poros A 20 and Operating Instructions publications provide product use details. Lastly, for more information on BAC’s product range please visit their web site. I hope this introduction was helpful and should you have questions please contact us at bp@lifetech.com Thanks for your time today Go to BAC: bacbv.com © 2012 Life Technologies Corporation. All rights reserved. The trademarks mentioned herein are the property of Life Technologies Corporation and/or its affiliate(s) or their respective owners. CaptureSelect is a registered trademark of BAC BV.