Identifying Genes Underlying Spinal Motoneuron Specification Karan Nathani SPUR 2011 Judith Eisen Lab Mentors: Liesl Van Ryswyk and Dr. Steve Seredick.

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Presentation transcript:

Identifying Genes Underlying Spinal Motoneuron Specification Karan Nathani SPUR 2011 Judith Eisen Lab Mentors: Liesl Van Ryswyk and Dr. Steve Seredick

Overview Objective Background Summer Research Conclusion Future Directions

Overview Objective Background Summer Research Conclusion Future Directions

Motoneuron Specification Goal: Understanding how motoneurons are specified Types of Neurons

Implications of This Study Treatment of several Motoneuron Diseases Spinal Muscular Atrophy patient

Why Zebrafish? Vertebrates Transparent Genetic Tools

Overview Objective Background Summer Research Conclusion Future Directions

Road to Olfactomedin 1a Microarray screen Signaling Molecule Axon Pathfinding

Olfactomedin 1a Background Nakaya and Tomarev 2007

Overview Objective Background Summer Research Conclusion Future Directions

Hypothesis Olfactomedin 1a plays a role in specifying the formation of motoneurons. Questions Olfactomedin’s Role in Motoneuron Specification? Olfactomedin’s Function?

Methods RNA in situ hybridization MO Injections PCR

Assessing Morpholinos Titration Concentrations: 50, 125, and 500 µM 50µM 125µM 500µM

Overview Objective Background Summer Research Conclusion Future Directions

Results Uninjected Transgenic FishMO injected transgenic fish

Results Conclusion: Olfactomedin 1a may have a role in axon pathfinding! Uninjected transgenic fishMO injected transgenic fish

Overview Objective Background Summer Research Conclusion Future Directions

Determine RNA Expression Pattern Further Studies of Protein Functions

Acknowledgements Eisen Lab Judith Eisen Liesl Van Ryswyk Steve Seredick Julia Ganz Levi Simonson Ellie Melancon Dayna Lamb Fish Facility Spur Program Peter O’Day Lyndsey Goforth Blakely Strand Toby Fish Interns

Thank you!

Splice-blocking Morpholino

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