BIOLOGYofCOMPLEMENT. BIOLOGYDIAGNOSIS COMPLEMENT.

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Presentation transcript:

BIOLOGYofCOMPLEMENT

BIOLOGYDIAGNOSIS COMPLEMENT

=C4d

= C3injury

= C4d C3injury MBL Apoptotic CMV cells CRP

= C4d C3injury MBL Apoptotic CMV cells CRP Regulators

= C4d C3injury MBL Apoptotic CMV cells CRP Regulators protection

C1 C4 C2 C3 MBL B C5 Classical Lectin Alternative Three Pathways of Complement Activation

C1 C4 C2 C3 MBL B C5 Classical Lectin Alternative Three Pathways of Complement Activation a a a b b b b a

C1C2C5C6C7C8C9 C4 C3 0.6 mg/ml 1.3 mg/ml C4bC2a C3bBb

C1C2C5C6C7C8C9 C4 C3 0.6 mg/ml 1.3 mg/ml C1inh Factor I C4bp CR1 MCP Factor I DAF CR1 MCP C4bp Factor I DAF CR1 Factor H CD59 Clusterin Vitronectin C4bC2a C3bBb

C3b > C4b CR1 iC3b > iC4b CR3 C3d > C4d CR2 FUNCTION

C4d vs C3d STAINING

ATGAM Thymoglobulin (Horse polyclonal) (Rabbit polyclonal) C4d+ / Total C4d Deposition ATGAM vs Thymoglobulin 9 / 9 0 / 8

C Reactive Protein Activation of Complement

ANTIBODY ASSOCIATED REJECTION 7 years after transplantation anti-DR7 and DRw53

ANTIBODY ASSOCIATED REJECTION after plasmapheresis plus IVIg anti-DR7 and DRw53 undetectable C4dC3d

Clearance of iC3b and C3d from Human Endothelial Cells in vitro

“SUBLYTIC” MAC LEUKOCYTE ACTIVATION BY

MAC Endothelial ACTIVATION OF ENDOTHELIAL CELL BY MAC W-P

MAC Endothelial ACTIVATION OF ENDOTHELIAL CELL BY MAC P-selectin vWfCD63 Tissue Factor

MAC Endothelial ACTIVATION OF ENDOTHELIAL CELL BY MAC P-selectin vWfCD63 Tissue Factor Synthesis PAF MCP-1 bFGF P-selectin Tissue Factor PDGF IL-8

MAC Endothelial P-selectin vWfCD63 Tissue Factor Synthesis PAF MCP-1 bFGF P-selectin Tissue Factor PDGF IL-8 Monocyte/ Macrophage Patelet P-selectin bFGF PDGF vWf Tissue Factor TNF 

B. Wasowska et al 2001 Transplantation 71, 727. vWf

Ig Knock Out Recipients

Tan et al. Arth & Rheum 40: , :3201:1601:801:40 Operating Characteristics of ANA Immunofluorescence Assays

HUMORAL IMMUNITY IN CARDIAC TRANSPLANTS Cardiac Surgery: J. Conte Cardiology: D Carter, EK Kasper, D Judge Immunogenetics: D Lucas, MS Leffell, AA Zachary Bioengineering: J Haun, R Alevriadou Pathology: RH Hruban, ER Rodriguez, WM Baldwin III