PHYLOGENETIC ANALYSIS OF A NEW GROUP OF CUCUMBER MOSAIC VIRUS ISOLATES BASED ON 2B GENE SEQUENCING Nesmelov I.B., Gnutova R.V., Tolkach V.Ph.

History of question was described for the first time by Selby at 1903 The 60 strains of this virus are infecting about 500 plant species from 67 families in nature CMV strains are divided into two groups according to the differences in their genomes and biological features More than 40 isolates was described at Primorye region On the base of serological assay isolates from Primorye were ascribed to Far Eastern serotype at 2008 orchid isolates was ascribed to Eastern Asian isolates

Aim The aim of our work is to show are the examined orchid isolates came from abroad or they are belonging to natural Far-Eastern CMV population

Virus structure spherical form size ~29 nm 32 morphological subunits of coat protein in nodes of crystal lattice contain 3 molecules of (+) RNA

Methods Infected plant Total RNA Sequencing Nicotiana tobacum cv Xanthi by Dreyper (1991) Isolation RT-PCR primersequence 5`-3`lenght, b Annealing temp. °C Length of product, bp FATGGAATTGAACGAAGGCGC RTGGGCGGAAGGGTCATTTTGA20 was made by “Sintol” company

Phylogenetic analysis South Korea China India China Far-Eastern China East-Asian isolates

Genetic distances JpSKEurChIndUSAFE Jp SK0.141 Eur Ch Ind USA FE Outgroup Jp – Japan, SK – South Korea, Eur – Europe, Ch – China, Ind – india, FE – Far-Eastern isolates

At present time we have separate population of CMV on orchid plants on Far-Eastern territory of Russian Federation. On both genetic distances and phylogenetic reconstruction Far-Eastern isolates are part of East-Asian Isolates. Conclusion

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