HPLC Bulk Property Detectors Refractive Index Detector
HPLC Bulk Property Detectors Refractive Index Detector Plus: 1) Measures a bulk property 2) Nearly Universal (different RI than mobile phase) 3) Comparable response for different analytes 4) Detects species with no chromophores Minus: 1) Temperature dependent 2) Poor sensitivity (LOD ≈ 100 ng) 3) No gradient elution
HPLC Bulk Property Detectors Evaporative Light Scattering Detector Solid state laser
HPLC Bulk Property Detectors Evaporative Light Scattering Detector Plus: 1) Measures a bulk property 2) Nearly Universal (must be non-volatile) 3) Does not detect liquids (gradients are ok) 4) Detects species with no chromophores Minus: 1) Signal not linear with concentration 2) Fair sensitivity (LOD ≈ 1 ng) 3) No salts or buffers in mobile phase
HPLC Bulk Property Detectors Conductivity Detector
HPLC Bulk Property Detectors Conductivity Detector Plus: 1) Measures a bulk property 2) Nearly Universal (must be ionic) 3) Common for Ion Exchange LC 4) Detects species with no chromophores 5) Simple, robust Minus: 1) Fair sensitivity (LOD ≈ 1 ng) 2) No salts or buffers in mobile phase 3) Gradients a challenge
HPLC Specific Property Detectors Conventional UV-Vis Absorption Detector Photodiode 1 Photodiode 2 Hg Pen Lamp 254 nm
HPLC Specific Property Detectors Multi-wavelength UV-Vis Absorption Detector Deuterium Lamp Photodiode Array
HPLC Specific Property Detectors Multi-wavelength UV-Vis Absorption Detector
HPLC Specific Property Detectors Multi-wavelength UV-Vis Absorption Detector Plus: 1) Measures a specific property 2) Nearly Universal (must have chromophore) 3) Most common of all detectors (~75%) 4) Potential to provide qualitative info. 5) Simple, robust Minus: 1) Fair sensitivity (LOD ≈ 1 ng) 2) Expensive with PDA, limited with Hg Lamp 3) Misses some important analytes
HPLC Specific Property Detectors Fluorescence Detector
HPLC Specific Property Detectors Fluorescence Detector
HPLC Specific Property Detectors Fluorescence Detector Plus: 1) Measures a specific property 2) Highly Selective (must fluoresce) 3) Second most common of all detectors (~15%) 4) High sensitivity (LOD ≈ 0.01 ng) 5) Can interrogate very small volumes Minus: 1) Not Universal 2) Limited Applications
HPLC Specific Property Detectors Electrochemical Detector
HPLC Specific Property Detectors Electrochemical Detector Plus: 1) Measures a specific property 2) Highly Selective (depends on reduction potential) 3) High sensitivity (LOD ≈ 0.01 ng) Minus: 1) Not Universal 2) Must have electrolyte in mobile phase 3) Mobile phase must be aqueous 4) Gradients not possible
HPLC Element Specific Detectors ICP Detector Detector
HPLC Element Specific Detectors ICP Detector Detector Plus: 1) Can be Highly Selective 2) Can be nearly Universal 3) Can have single sensitivity for all analytes Minus: 1) $$$ 2) Mobile phase must be aqueous 4) Mobile phase must not contain element of interest
UV-Vis detection of sugars in juice
UV-Vis detection of sugars in juice
ICP Carbon detection of sugars in juice
ICP Carbon detection of sugars in orange juice
ICP Carbon detection of amino acids
ICP Carbon detection of amino acids
HPLC Derivatization Detectors Tag analytes with fluorophore Before or After Separation
HPLC Derivatization Detectors Tag analytes with fluorophore Before or After Separation
Detect the change in a constant signal HPLC Indirect Detectors Detect the change in a constant signal as the analyte passes
Hollow Cathode Detector Recent HPLC Detector Hollow Cathode Detector
Hollow Cathode Detector Recent HPLC Detector Hollow Cathode Detector
Hollow Cathode Detector Recent HPLC Detector Hollow Cathode Detector
Hollow Cathode Detector Recent HPLC Detector Hollow Cathode Detector
Hollow Cathode Detector Recent HPLC Detector Hollow Cathode Detector
Hollow Cathode Detector Recent HPLC Detector Hollow Cathode Detector