parts.mit.edu iGEM & Synthetic Biology International Genetically Engineered Machine Competition Randy Rettberg igem2007.comparts.mit.edu SB 3.0 Zurich 6/24/07

parts.mit.edu An Engineering Question Can simple biological systems be built from standard, interchangable parts and operated in living cells? Or, is biology so complex that each case is unique?

parts.mit.edu Education Driving Research

parts.mit.edu International Genetically Engineered Machine Competition 2005 −13 Schools CaltechMITUC Berkeley Cambridge, UKOklahomaUCSF DavidsonPenn StateUT Austin ETH, ZurichPrinceton HarvardToronto, Canada −Sponsored by Microsoft/MIT iCampus

parts.mit.edu iGEM Schools, 33 Teams 450 participants Europe, Canada, Asia, Latin America, US Some EU funding in place iGEM ambassadors for US, Asia, and Europe Jamboree November 4 & 5 Supported by Microsoft Research and iCampus

parts.mit.edu Ege University, Turkey iGEM 2006

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parts.mit.edu Meet the Teams

parts.mit.edu iGEM Site Valencia iGEM Site

parts.mit.edu iGEM 2006 Results

parts.mit.edu Sample iGEM Projects  BerkeleyControlled Communications  DavidsonFlipping DNA  HarvardDNA Boxes  ETH ZurichPattern Matching  LjubljanaControlling Sepsis  Chiba JapanLight-Directed Chemotaxis  MississippiHydrogen Gas Detector  MITBanana and Wintergreen Smells  EdinburghArsenic Detector

parts.mit.edu MIT iGEM 2006 eau d’e coli mit igem 2006

The field test device A test tube could contain all the necessary components: Freeze dried bacteria, growth medium, indicator powder, Ampicillin salt, etc … These tubes could then be given to local villagers to monitor their own water quality themselves A good alternative to the widely used Gutzeit method

parts.mit.edu Some Engineering Answers Can simple biological systems be built from standard, interchangable parts and operated in living cells? - YES, SOMETIMES Or, is biology so complex that each case is unique? - NOT ALWAYS

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parts.mit.edu Financial Resources Small Sample: $20K8 $30K8 $40K1 $50K4 >$50K2

parts.mit.edu 2008: 100 Schools -> Partition? Geographic Competitions Tracts Health and Medicine Energy and the Environment Parts and Devices Fun and Games Sensors Other iGEM 2007 and Beyond

parts.mit.edu The Impact of iGEM Students Making future synthetic biologists Teaching graceful competition Instructors Opportunities for junior faculty New programs - new ideas A task worth the effort Schools Synthetic biology entering curriculum Energize research programs Synthetic Biology Examples, parts, successes, testamonials

parts.mit.edu Registry of Standard Biological Parts An industry based on standard parts requires catalogues and suppliers of those parts.

parts.mit.edu Registry Registry Parts Catalog

parts.mit.edu Registry Contents 7/2007 Promoters Protein Coding Reporters RNA Terminators Signaling Many project parts 1400 Parts Available as DNA

parts.mit.edu Constitutive Promoters - Berkeley Part Characterization

parts.mit.edu DNA Repository

parts.mit.edu DNA Repository All parts sent to teams Parts are from previous teams

parts.mit.edu Robotic Assembly

parts.mit.edu DNA Synthesis Offer GENEART synthetic DNA offer for iGEM teams All you want at $0.70 per base 100,000 bases total at $0.25 per base About 2000 bases per team for $500 GENEART is a primary sponsor of iGEM

parts.mit.edu New Programs BioBrick Part Program iGEM in Europe Registry in Europe New Registry Workshop Standards Workshop iGEM Steering Committee

parts.mit.edu Standards Device Families Protein : DNA logic Post-translational control RNA-based control Localization Measurement Input and Outputs (PoPS - Polymerase Per Second) Genetic stability Cross-lab consistency Operating Environment Load on the cell Environmental impact on performance Models

parts.mit.edu Sponsors National Science Foundation SynBERC CSBi GENEART Microsoft Research Biological Engineering - MIT

parts.mit.edu Teachers Workshops MIT May 26 th China June Zurich June 27 th Hands-on training Future instructors welcome

parts.mit.edu How can I participate? Organize a team for 2008 Volunteer for the BioBrick parts program. Join the Awards committee Be a judge Have your lab join the Registry Contribute parts to the Registry Use BioBrick part standards Help raise money for teams and ambassadors in your area. Join iGEM committees (see the wiki) Join iGEM in Europe