Genetic Engineering What we can do with genes

Restriction Enzymes: The Video Clip

Types of Genetic Engineering 1. Recombinant DNA Technology Inserting gene from one organism into another (usually bacteria) Inserting gene from one organism into another (usually bacteria) Ex: Inserting human insulin gene into bacterium; bacterium will now make insulin Ex: Inserting human insulin gene into bacterium; bacterium will now make insulin

Recombinant DNA: You Tube Video Recombinant DNA: You Tube Video

Types of Genetic Engineering 2. PCR (Polymerase Chain Reaction) Replicating a DNA sequence over and over and over and over again… Replicating a DNA sequence over and over and over and over again… Allows scientists to study specific sequences Allows scientists to study specific sequences Applications of this include: mapping of the human genome project, single sperm analysis, molecular archaeology and ancient DNA, molecular ecology and behavior, disease diagnosis and drug discovery. Applications of this include: mapping of the human genome project, single sperm analysis, molecular archaeology and ancient DNA, molecular ecology and behavior, disease diagnosis and drug discovery. Let’s See!

Gel Electrophoresis: The Video Clip

Types of Genetic Engineering 3. Gel Electrophoresis Separates DNA into different banding patterns based on what they contain (HOW MANY BASES IN EACH STRAND!) Separates DNA into different banding patterns based on what they contain (HOW MANY BASES IN EACH STRAND!) DNA separates due to opposite charge at one end of chamber and how heavy it is DNA separates due to opposite charge at one end of chamber and how heavy it is Makes a “finger-print” Makes a “finger-print” Parents will have similar banding to their children and vice-versa Parents will have similar banding to their children and vice-versa Let’s See! Let’s See!

Benefits of Genetic Engineering 1. Making large amounts of a human protein, such as insulin 2. Finding cures by altering DNA 3. DNA Fingerprinting has helped us to see how similar certain orgs. are 4. Alter DNA in plants to make them resistant to certain diseases 5. Paternity tests! Let’s See!Let’s See!