DNA: Transcription & Translation How do we go from DNA to PROTEIN?

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Presentation transcript:

DNA: Transcription & Translation How do we go from DNA to PROTEIN?

WHAT DO WE NEED? DNA RNA –mRNA: messenger RNA –rRNA: ribosomal RNA –tRNA: transfer RNA Amino Acids  Proteins

DNA The genetic code or “blue-print” of life Contains the bases A, T, C, and G to code for amino acids

DNA/ Genes/ Codons DNA is made of approximately 80,000 genes Genes are sections of DNA that code for a single protein Codons are a set of 3 bases that code for an amino acid

RNA Polymer of ribonucleotides –Sugar (ribose) –Phosphate group –Nitrogenous base (A, U, C, G) Single-strand Three types: mRNA, rRNA, tRNA

ROLES OF RNA mRNA: transports information from DNA from the nucleus to the cell’s cytoplasm rRNA: (makes up ribosomes): clamps on to mRNA and reads its information to assemble amino acids in the correct order tRNA: transports amino acids to the ribosomes to be assembled into proteins

Transcription & Translation

TRANSCRIPTION Process in the cell’s nucleus where enzymes make an RNA copy (mRNA) of the DNA strand

Steps of Transcription Separation of Strands: enzymes unzip DNA in the bases Base Pairing: free RNA ribonucleotides pair with complementary DNA bases –Only happens to ONE strand of DNA –Uracil bonds with Adenine of the DNA

Steps of Transcription (cont’d) Ribonucleotides Bond: ribonucleotides of mRNA bond due to an enzyme Leaves Nucleus: mRNA strand breaks away from DNA and enters the cytoplasm (where translation begins)

Transcription to Translation

Translation Process of converting information in mRNA into a sequence of amino acids that will make a protein

Steps of Translation Ribosomes Attach: mRNA enters the cytoplasm where ribosomes of rRNA attach to it rRNA reads mRNA: rRNA slides down the mRNA and signals to tRNA to bring the right amino acid –Start codon (AUG) signals where to begin making the protein

tRNA tRNA has a clover- leaf shape with an anti-codon attached to one of the “leaves” and an amino acid attached to the “stem”

Steps of Translation (cont’d) tRNA brings amino acids: tRNA with the correct anti-codon arrives and temporarily bonds to the codon of mRNA rRNA slides and reads: rRNA continues to slide down the mRNA and reads next codon  new tRNA

Steps of Translation (cont’d) Amino acids join: an enzyme joins the amino acids (of tRNA) with peptide bonds –Once joined, the now amino acid free tRNA detaches from the mRNA strand –This process continues until a stop codon (UAA/UAG/UGA) is read

Transcription & Translation: END RESULT A chain of amino acids joined by peptide bonds  PROTEIN

Transcription & Translation Sequence Animation of Protein Synthesis

Protein Synthesis Video

References Transcription & Translation Images: ans.HP.html ans.HP.html Transcription & Translation Animation: Protein Synthesis Video: Messenger RNA, Transfer RNA, and Protein Synthesis. United Learning Discovery Education. 15 December 2009