Virus Replication Hsiao-Sheng Liu 劉校生國立成功大學微生物暨免疫學研究所.

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Virus Replication Hsiao-Sheng Liu 劉校生國立成功大學微生物暨免疫學研究所

The objectives: How to calculate virus titer? One step growth curve The general stages of viral replication cycle Attachment, penetration and release of the viruses

How to measure virus titer?

Baculovirus plaques Zones of clearing (plaques) are generated by infection of insect (Sf9} cells with individual baculovirus particles. Uninfected Sf9 cells surrounding the plaque are stained pink with neutral red.

PFU: plaque forming unit 1 PFU = 1 plaque = 1 bacterial phage Count : plaque 數量 x 稀釋倍數 x 10/9 = PFU/ml Ex: 211 x 10 7 x 10/9 = 2.34 x 10 9 PFU/ml 211 plaques

What is one step growth curve?

陰蔽期潛伏期

What are the general stages in viral replication cycles?

General replication procedure

Downloaded from: StudentConsult (on 15 May :15 AM) © 2005 Elsevier

Complicated replication procedure (e.g. Herpes virus)

How the viral replication cycle is known?

Attachment: receptor, tissue tropism

HA: Hemagglutinine

Trimmers of HA on influenza virus membrane

Coreceptor: CCR5 CRCX4

CCR5: macrophage-tropic HIV CXCR4: lymphocyte-tropic HIV

How does the virus enter the cell?

Endocytosis Fusion Pinocytosis (Viropexis)

Paramyxovirus fusion  Syncytia

Togavirus

How is the virus released from the cell?

Orthomyxovirus with M protein Herpes virus

Respiratory syncytial virus budding

Brooks et al, 1998