Bacterial Cultivation & Growth Culturing Microorganisms –Binary fission & sporulation –What determines growth? –Media Types –Batch vs Continuous Culture.

Slides:

Advertisements

Similar presentations

GROWTH OF CULTURE Population growth

Advertisements

Bacterial Generation Time

By: Mdm. Noor Amirah Abdul Halim BIOREACTION AND BIOREACTOR.

Endospores – a special resistant dormant structure, formed under periods of environmental stress

Chapter 4. Growth of cells

Microbial Growth.

A Tiny Bit of Microbiology EVE 430. Summary of nutritional groups.

1 Microbial growth: prokaryotic cell cycle and growth curve Chapter 6.

General Microbiology (Micr300) Lecture 4 Nutrition and Growth (Text Chapters: ; 6.1; ; )

Microbial Growth Chapter 6.

Microbial Growth. What do they need to grow? Physical needs –Temperature, proper pH, etc. Chemical needs –Molecules for food, ATP production, coenzymes,

Growth curves of micro-organisms. Learning Objectives  Discuss the growth curves of micro organisms  Outline the differences between batch and continuous.

Faculty of Medicine and Health Sciences Microbiology Lab second semester 2013 prepared by: Mohammad Al-Qadi

Measurement of Bacterial Growth

Cell Growth Kinetics Introduction

Growth and Multiplication of Bacteria

Microbial Nutrition and Growth Microbial Population Growth

Microbial Growth. Growth of Microbes Increase in number of cells, not cell size One cell becomes colony of millions of cells.

**Microbial Growth** Growth= an increase in the number of cells, not an increase in size Generation=growth by binary fission Generation time=time it takes.

Cells Growth in Continuous Culture Continuous culture: fresh nutrient medium is continually supplied to a well-stirred culture and products and cells are.

Growth and Cultivation of micro-organisms

Inorganic Nutrient Availability & Phytoplankton Growth: the start of Biogeochemical Cycles Re-read pp , Habitat Determinants of Primary Production.

Microbial Growth and Culture

Endospores and formation of cell aggregates. Endospores - metabolically dormant stages observed in certain Gram positive bacteria A survival strategy.

Warm-Up If a bacteria cell undergoes binary fission, producing an identical copy of itself, what happens to the original parent cell? Does it still exist?

Chapter 2 Physiology of Bacteria Section 1 and section 2(study by yourself)

LAB NO 8 LAB NO 8 Environmental Factors Affecting Microbial growth.

Bacteria are known for their rapid growth, many of the enterics will grow and then divide every 20 minutes under ideal conditions. Some bacteria isolated.

1 GROWTH AND CULTURING OF BACTERIA CHAPTER 6. 2 Growth and Cell Division Growth Binary fission.

Plot of increases in cell number vs time for cell dividing by binary fission = Growth Curve Logarithmic Growth N = No2 n N = No2 n Exponential Growth N.

Microbial Growth Binary Fission Growth Rate Generation Time E. coli can double every 20 minutes Many Bacteria have hr Generation Times.

Environmental Factors Tolerance to All Environmental Factors (Shelford’s Law of Tolerance) Temperature Solute Concentration / Water Activity pH (acidity.

Lecture 1: Kinetics of Substrate Utilization and Product Formation

Bacterial growth:. Bacterial Growth Curve: The schematic growth curve shown below is associated with simplistic conditions known as a batch culture. It.

Aerobic Respiration. Anaerobic Respiration Chemolithotrophic respiration.

Microbial Growth Chapter 4.

Typically refers to an increase in population rather than in size

Batch Growth Kinetics Heat generation by microbial growth

General Microbiology Laboratory Bacterial Generation Time.

Lecture 4 Dr. Dalia M. Mohsen Prof. of Microbiology.

Microbial Growth Growth= an increase in the number of cells, not an increase in size Generation=growth by binary fission Generation time=time it takes.

Food Microbiology 1 Unit 4 Microbial Growth. Bacteria are single-celled organisms Bacteria multiply in a process called binary fission in which two cells.

Characteristics and study of prokaryotic growth How do we grow bacteria in the laboratory? What is required for growth? How do we measure bacterial growth?

Structure of the bacterial cell VI- Bacterial Spore - Definition - Formation - Shape - Importance.

Introduction to Bacteriology

Dr Rita Oladele Dept of Med Micro &Para CMUL/LUTH

Bacterial growth The mathematics of bacterial growth is fairly simple, since each original cell divides to form two new cells, with the loss of the original.

Microbial Growth. Growth of Microbes Increase in number of cells, not cell size One cell becomes colony of millions of cells.

1 Growth and Cell Division Growth: –Not size, but number of cells Bacteria: –Binary fission Yeast: –Budding.

Structure of bacterial cell Surface structure: 1-Capsule 2- Cells wall…. Peptidoglycan 3- Cell membrane…. bilayer 4- Appendages Flagella and Pili.

Cell Growth Kinetics -Introduction -Growth patterns and kinetics in batch culture - growth phases - effect of factors: oxygen supply - heat generation.

Influence of Chemical and Physical Factors in Environment

Microbial Growth refers to increase in number of cells not in size.

Growth of Bacterial Culture

Batch and Continuous Flow

BACTERIAL GROWTH CURVE MONOD’S EQUATION MADE BY JITENDRA PRATAP SINGH M TECH ENV ENGG.

BACTERIAL GROWTH & REPRODUCTION

Microbial Growth.

Growth of bacteria Dr. Sahar Mahdi.

Microbial Growth Binary Fission Growth Rate Generation Time

Identification technique Bacterial Growth curve Bacterial reproduction

Growth curves of micro-organisms

MICROBIAL GROWTH CURVE

Growth of bacteria Dr. Sahar Mahdi.

Microorganisms & Biotechnology

Bacterial Growth and Reproduction.

Bioreactors Engineering

Microbial Growth and Nutrition

Growth curves of micro-organisms

Bacteria are known for their rapid growth, many of the enterics

Presentation transcript:

Bacterial Cultivation & Growth Culturing Microorganisms –Binary fission & sporulation –What determines growth? –Media Types –Batch vs Continuous Culture Growth

Binary Fission in Prokaryotes

Dividing vegetative cells But what happens when stressed or starved?

Endospores: Young (24 h)Old (96 h) free; sub-terminal; central; terminal Resting stage during “lean or stressful times”. Resistant protein coat! Develop in different locations of vegetative cell: Schaeffer – Fulton Stain:

Endospores & Sporulation Dipicolinic Acid & Calcium protects DNA from heat. Spore coat proteins resist toxic chemicals. exosporium (cortex)

Spore Germination: activation; germination; outgrowth

Culturing Microorganisms Tolerance to All Environmental Factors (Shelford’s Law of Tolerance) Growth Limiting Resource (Liebig’s Law of the Minimum): Nutrient in least supply relative to bacterial needs will cap growth yield. Temperature Solute Concentration / Water Activity pH (acidity versus alkalinity) Oxygen Concentration Barometric Pressure Electromagnetic Radiation

Closed (“batch”) Culture Systems “growth curve” Unbalanced Growth Balanced Growth: Rates of RNA = Protein = DNA = binary fission

Exponential Growth Phase N t = N o + 2 n Number of generations (n) = (log N t – log N o ) / log 2 Growth Rate Constant (k) = n/t It is expressed in units of generations per hours (h -1 ) Generation time (g) = 1/k; it is expressed in units of hours (h).

Time (hours) k = (log N t – log N o ) / t Generation time (g) = doubling time

Nutrient Concentration Effects in Batch Cultures: Total growth will increase until limiting nutrients are exhausted (included oxygen for aerobes) or metabolic byproducts accumulate that change environmental conditions to inhibit growth (toxicity). Growth rate will also increase with increasing nutrient concentration up to a some maximum value, beyond which there is no effect (transporters are saturated with there substrate.

Open (“continuous”) Culture Systems Chemostat: growth rate = dilution rate (D = f/V); constant dilution rate with nutrient limiting growth. Turbidostat: dilution rates varies to maintain constant turbidity (cell density); no limiting nutrient. Flow (f) Volume (V) Wash out!