Present by Pitchvipa Prommas Advisor Dr. Apinan Soottitantawat Co-advisor Dr. Uracha Ruktanonchai Encapsulation of menthol in rice starch by spray drying 1

Contents Introduction Literature reviews Objectives Experimental Scopes of research 2

Introduction 3

4 Cooling Powder

Menthol Ref. 5

6 Talcum Magnesium Silicate or [Mg 3 Si 4 O 10 (OH) 2 ]

7 Commercial cooling powder Talcum is toxic Menthol is high volatile

Rice starch Ref. Thailand has 20,194 tons of rice starch in each year 8

9 Encapsulation core High temperature o2o2 Humidity

Encapsulation Fluidized Bed Coacervation Spray Drying Encapsulation 10

Encapsulation Coacervation Spray Drying Fluidized Bed 11 core wallcoating

Spray drying Low cost Text Encapsulation Simple method 12 Easy to scale up

Spray Dryer 13 Ohkawara type L-8 Heater Inlet air temp. ( ºC) Atomizer (30,000 rpm) Cyclone Pump (25 ml/min) Drying chamber Powder Outlet air temp. (65-70ºC) Feed liquid 1. Atomization of liquid feed 2. Evaporation of water 3. Powder separation by cyclone

Experimental 14

Experimental Preparation of encapsulated menthol powder Analysis of menthol powder 15

8000 rpm, 2 min 8000 rpm, 1 min -Inlet temp  C -Flow rate 25 and 35 ml/min -Atomizer speed rpm Preparation of encapsulated menthol powder Melted menthol Rice starch -Casein as surfactant -Maltodextrin -Distilled water

- Morphology by SEM JSM-6060 encapsulated powders 17 Analysis of menthol powder Ref.

encapsulated powders 18 Analysis of menthol powder -Menthol content by GC-2014 Retention (%) = R2 x 100 R1 R1 = initial menthol content in feed R2 = menthol content retained in the powder product

19 encapsulated powders Analysis of menthol powder - Water content by vacuum dryer ADP-21 Water content ＝ W1-W2-[M1-M2] W1 = weight of encapsulated powder before drying W2 = weight of encapsulated powder after drying for 20 hr M1 = weight of menthol in encapsulated powder before drying M2 = weight of menthol in encapsulated powder after drying for 20 hr

20 Expected results

Applying the encapsulation technique for cosmetic industry or pharmaceutical industry Applying the encapsulation technique for cosmetic industry or pharmaceutical industry Getting long lasting cooling powder 21

22 Results

Improving retention by adding casein and maltodextrin Comparison with commercial powder 23 -Inlet temperature -Feed flow rate -Atomizer speed Spray drying parameter

24 Effect of surfactant

25 Retention (%) Emulsion size (μm) control with casein 5% Menthol 20 wt % dry basis

26 Menthol 20 wt % dry basis ControlWith casein 5%

Effect of maltodextrin content

Maltodextrin Content (%) Retention (%) Particle size (μm) Water content (%) Casein 5 wt%, Menthol 10 wt% dry basis

MD = 0% MD = 10% MD = 5% MD = 20% Casein 5 wt %, Menthol 10 wt % dry basis

30 MD = 0%MD = 5%MD = 10%MD = 20% Casein 5 wt%, Menthol 10 wt % dry basis

Emulsion size distribution MD = 0% MD = 5% MD = 20% MD = 10%

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Effect of inlet temperature

Inlet temperature ( ° C) Retention (%) Particle size (μm) Water content (%) Casein 5 wt%, Menthol 10 wt%, Maltodextrin 10 wt%

120 ° C 140 ° C160 ° C 180 ° C Casein 5 wt%, Menthol 10 wt%, Maltodextrin 10 wt%

° C 140 ° C160 ° C 180 ° C Casein 5 wt%, Menthol 10 wt%, Maltodextrin 10 wt%

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38 Emulsion size distribution 120 ° C140 ° C 160 ° C180 ° C

Effect of feed flow rate

Feed flow rate (ml/min) Retention (%) Particle size (μm) Water content (%) Casein 5 wt%, Menthol 10 wt%, Maltodextrin 10 wt%

41 Emulsion size distribution Flow 35 ml/min Flow 25 ml/min

Flow 35 ml/min Casein 5 wt%, Menthol 10 wt%, Maltodextrin 10 wt%

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44 Effect of atomizer speed

45 Atomizer speed(rpm) Retention (%) Particle size (μm) Water content (%) Casein 5 wt%, Menthol 10 wt%, Maltodextrin 10 wt%

rpm30000 rpm Casein 5 wt%, Menthol 10 wt%, Maltodextrin 10 wt%

47 Emulsion size distribution rpm30000 rpm

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49 Comparison between encapsulated powder and commercial powder

50 Sample mg menthol/ g powder Powder size (μm) Morphology Encapsulated powder Commercial powder

Conclusion 51

52 Conclusion Adding maltodextrin can get more retention. Spray drying parameter ► Lower inlet temperature ► Lower feed flow rate ► Higher atomizer speed

Thank You Q&A 53