UV–Vis Molecular Absorption Spectrometry Chapter 13.

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Presentation transcript:

UV–Vis Molecular Absorption Spectrometry Chapter 13

Measurement of Transmittance and Absorbance

Absorption Cell

Effect of Polychromatic Radiation

Instrument Noise – Effect on Transmittance and Absorbance

Molecular Absorption Measurements Components

Output from a Deuterium Lamp

Typical Sourses of Light Deuterium Lamp Tungsten Lamp

Blackbody Radiation Curves

Spectral Bandwidth

Effect of Slit Width

Slit Width and Resolution

Resolution

Interference Filter

Transmission Characteristics of Transmission Filter

Absorption vs. Interference Filter

Prism Monochromator

Grating Monochromator

Echellette Grating

Dispersion for Three Types of Monochromator

Combination Absorption Filter

Photon Transducers

Phototube

Photomultiplier

Single-Beam Instrument

Double-Beam Instrument beams separated in space

Double-Beam Instrument beams separated in time

Single- and Double-Beam Photometer

Spectronic 20

Double-Beam Spectrophotometer UV-Vis

Double-Beam Recording Spectrophotometer UV-Vis

Double-Dispersing Spectrophotometer

Multichannel Spectrophotometer grating and photodiode array transducer

Multichannel Diode Array Spectrometer

End

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