Vitamin C Determination in Various Fruit Juices Over Time Andrew Mullenax Sam Yatzkan.

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Presentation transcript:

Vitamin C Determination in Various Fruit Juices Over Time Andrew Mullenax Sam Yatzkan

Instrumentation UV-Vis LC/MS HPLC

Procedure Samples diluted – 1:10 (v/v) Room Temperature Half Opened, Half Closed Vacuum Filtered-Samples with visible solids

Samples Dole Brand Simply Lemonade Brand

Ascorbic Acid Standards 5, 10, 20, 30ppm Spiked Samples Characterized Wavelength: 262nm HPLC: Retention Time of ~1.6 minutes – Solvent Mixture of.50mL/min degassed H 2 O and Methanol LC/MS: Retention Time of ~1.6 minutes – Solvent Mixture of.75mL/min degassed Methanol and.25mL/min degassed H 2 O

Data – UV-Vis Samples Day 1 Characterized Wavelength Day 2 Characterized Wavelength (nm) Day 3 Characterized Wavelength (nm) Day 4 Characterized Wavelength (nm) Cran-Apple Juice Closed Pineapple Juice Closed White Grapefruit Closed Lemonade Closed Cran-Apple Juice Opened Pineapple Juice Opened White Grapefruit Opened Lemonade Opened232281

HPLC Calibration Curve

Data - HPLC SamplesDay 1 Concentration (ppm)Day 2 Concentration (ppm)Day 3 Concentration (ppm)Day 4 Concentration (ppm) Cran-Apple Juice Closed Pineapple Juice Closed White Grapefruit Closed Lemonade Closed Cran-Apple Juice Opened Pineapple Juice Opened White Grapefruit Opened Lemonade Opened

LC/MS Calibration Curve

Data – LC/MS Samples Day 1 Concentration (ppm) Day 2 Concentration (ppm) Day 3 Concentration (ppm) Day 4 Concentration (ppm) Pineapple Juice Closed Lemonade Closed Cran-Apple Closed White Grapefruit Closed Pineapple Juice Opened Lemonade Opened Cran-Apple Juice Opened White Grapefruit Opened

Conclusion Results not very reliable since we had varying retention times Did not get very good separation in chromatographs of the samples Too difficult to tell whether or not vitamin c concentration actually decreased over time

Improvements To Be Made Better solvent system Better/Different Instrument Settings Dilute more Vacuum Filter All Samples Regardless

References Lee, H. S. HPLC Method for Separation and Determination of Nonvolatile Organic Acids in Orange Juice. JAFC, 1993, 41(11), pp Nisperos-Carriedo, M. O.; Buslig, B. S.; Shaw, P. E. Simultaneous Detection of Dehydroascorbic, Ascorbic, and Some Organic Acids in Fruits and Vegetables by HPLC. Chem. Anal. 1992, 40(7), pp Silva, F. O.; Total ascorbic acid determination in fresh squeezed orange juice by gas chromatography. Food Control, 2005, 16(1), pp V., K.; D., S.; E., M. Ascorbic acid content of commercial fruit juices and its rate of loss upon storage. Food Chemistry, 2000, 70(3), pp