Optical Tweezers at The National Institute of Standards and Technology and Davidson College Research performed by: Rachel Patton McCord.

Slides:

Advertisements

Similar presentations

Biology Journal Ch 3.

Advertisements

These figures correspond to the image using shear =10 pixels And 2D convolution was done in order to get the images Using R=0 y0 Using R=1 y1.

1 Outline Basic Idea Simple Theory Design Points Calibration of Forces Selected Biological Applications.

Limits and Interfaces in Sciences / Kumboldt-Kolleg São Paulo-SP,

Biophysics of macromolecules Department of Biophysics, University of Pécs.

PowerPoint Learning Quest Biology 9 Unit 5: Cell Biology (Part 2) Created by: Jeff Wolf and Mike Graff.

Single Molecule Studies of DNA Mechanics with Optical Tweezers Mustafa Yorulmaz Koç University, Material Science and Engineering.

Team: Maryam Badakhshi, Shannon O’Keefe, Laura Poloni, Hasmita Singh.

Optical Tweezers F scatt F grad 1. Velocity autocorrelation function from the Langevin model kinetic property property of equilibrium fluctuations For.

Optical Diagnostics Thomas Tsang tight environment high radiation area non-serviceable area passive components optics only, no active electronics back.

Development of a thermal cooling system for a PMT (Photo Multiplier Tube) Summer Researcher Yasmine Salas Colorado State University Advisor: Dr. Julia.

Methods: Single-Molecule Techniques Biochemistry 4000 Dr. Ute Kothe.

Microscopy, Staining, and Classification

1.Lasers: What they are & how they work; Laser tweezers: moving things with light without touching; Laser applications: science, technology, & everyday.

Lecture 1 Rob Phillips California Institute of Technology (Block et al.) (Wuite et al.)

A Biomechanical Comparison of Cancerous and Normal Cell Plasma Membranes Olivia Beane Syracuse University BRITE 2009.

Optical Tweezers + DNA Stretching Lecture Notes stored at: Then select the “Lecture” button. David Carberry,

Example: A 20 kg block is fired horizontally across a frictionless surface. The block strikes a platform that is attached to a spring at its equilibrium.

“Nanophotonics and Optical Control of Single Nanoparticles” Keonwoo Nam Moscow 2012 Supervisor: Professor A. A. Fedyanin Lomonosov Moscow State University,

1 Optical Tweezers rolf. 2 Project Goals We will calibrate the strength of an optical trap (Optical Tweezer) Optical Tweezers may be used to measure very.

Using Optical Tweezers as a Tool in Undergraduate Labs. Paul Ingram, Ido Braslavsky and David F. J. Tees Dept of Physics and Astronomy, Ohio University,

An Introductory Information about Optical Tweezers Mustafa Yorulmaz Koç University, Material Science and Engineering.

Healthcare&Photonics quantitative optical trapping Tu LOGO.

Project Overview Laser Spectroscopy Group A. A. Ruth Department of Physics, University College Cork, Cork, Ireland.

Team: Maryam Badakhshi, Shannon O’Keefe, Laura Poloni, Hasmita Singh.

Optical Tweezers Sarah Nichols March 17, Outline Motivations Operation Layout Successes and Difficulties Future Directions.

Copyright © 2009 Pearson Education Inc., publishing as Pearson Benjamin Cummings Lecture prepared by Mindy Miller-Kittrell, University of Tennessee, Knoxville.

Evaluating cell matrix stiffness with a multiphoton confocal microscope-optical tweezer setup Berney Peng, Carlo Alonzo, Lawrence Xia, Lucia Speroni, Irene.

Theoretical Study of the Optical Manipulation of Semiconductor Nanoparticles under an Excitonic Resonance Condition + Reference + T.Iida and H.Ishihara,

Jeopardy Organelles The Microsco pe Living OrCell Mem Organelles Again Q $100 Q $200 Q $300 Q $400 Q $500 Q $100 Q $200 Q $300 Q $400 Q $500 Final Jeopardy.

Nicholas DiPreta.  Science of light  How light behaves and interacts with matter.

Use of tethering for axial confinement in optical tweezers Mark Cronin-Golomb Biomedical Engineering Tufts University.

Advanced Biology Visualizing Cells. The Human Eye  Resolution – The minimum distance two points can be apart and still be distinguished as two separate.

Chapter 8 Potential Energy. Potential energy is the energy associated with the configuration of a system of objects that exert forces on each other This.

Dr Gihan Gawish King Saud University Riyadh Saudi Arabia 1.

Created by: Maryam Badakhshi, Shannon O’Keefe, Laura Poloni, Hasmita Singh.

Single Molecule: Detection and Manipulation Ilan Tsafrir Tamar Arzi Physics of Complex Systems - Weizmann Institute of Science Interaction Between Membranes.

Detection of Heteroplasmic Mitochondrial DNA in Single Mitochondria Summary and Discussion Mustafa Albahrani.

Potential Energy Potential energy can also be stored in a spring when it is compressed; the figure below shows potential energy yielding kinetic energy.

Transverse optical mode in a 1-D chain J. Goree, B. Liu & K. Avinash.

Trap laser (6 mW - 1 cm diameter) x 6 -  /2 detuned to 171Yb 1P1 oven Zeeman slower Trapped atom number Lens f=7.5 cm, diameter d=2.54 cm PMT = 5

CONSTRUCTION OF A RAMAN TWEEZER SYSTEM FOR INVESTIGATION OF BIOLOGICAL MOLECULES Günay Başar İstanbul Teknik Üniversitesi International.

Team: Maryam Badakhshi, Shannon O’Keefe, Laura Poloni, Hasmita Singh.

Wind. Review  What is a convection cell?  An area where high and low energy molecules move in a circular pattern rising when they gain energy and falling.

3.052 Nanomechanics of Materials and Biomaterials Prof. Christine Ortiz DMSE, RM Phone : (617) WWW :

Cell membrane experiments Dept. of Experimental Orthopaedics and Biomechanics Bioengineering Reza Abedian (M.Sc.)

 Bright-field  Dark-field  Phase Contrast  Fluorescence.

Date of download: 6/25/2016 Copyright © 2016 SPIE. All rights reserved. Schematic optical layout of the instrument. Color box legend: Upright optical tweezers.

Date of download: 6/27/2016 Copyright © 2016 SPIE. All rights reserved. Schematic of the experimental setup. (1) Nd:YVO4 laser. (2) Beam expander. (3)

By c.Keerthana.  First described by Dutch physicist frits Zernike in  It is a type of light microscopy.  It is a contrast enhancing optical technique.

4/7/11 Robert Kim, BME, AMS Steven Su, BME, AMS Saurabh Vyas, BME, EE Mentors: Dr. Emad Boctor, Dr Russell Taylor Special Thanks to: Nathanael Kuo Interventional.

Development of a 3-D Fibre Based Laser Light Force Optical Trap

C-BER - Centre for BIOMEDICAL ENGINEERING RESEARCH

Volume 84, Issue 6, Pages (June 2003)

Optical Diagnostics tight environment high radiation area

Determination of Intercellular Calcium Concentrations in Cardiac Myocytes Using Fluorescence and a Single Fiber Optic Method Paul Clark, Martin Garcia,

July 13, 2004 Summarized by Ji-Yoon Park

İstanbul Teknik Üniversitesi

LASER PHOTODETACHMENT SPECTROSCOPY OF THE S2- ION

Volume 96, Issue 1, Pages (January 2009)

Physics Review Optical Power Topic Slides Minutes Displacement Vectors

Levels of Organization

Real-Time Measurement of Spontaneous Antigen-Antibody Dissociation

Volume 88, Issue 6, Pages (June 2005)

Introduction to Biophysics Lecture 17 Self assembly

Determination of Intercellular Calcium Concentrations in Cardiac Myocytes Using Fluorescence and a Single Fiber Optic Method Paul Clark, Martin Garcia,

Find the limit {image} ,024 2,160 -1,

Alex King, Nathan Jordan, Dr. Oleksiy Svitelskiy

Semiconductor Double Quantum Dot Maser

Presentation transcript:

Optical Tweezers at The National Institute of Standards and Technology and Davidson College Research performed by: Rachel Patton McCord

Background: Optical Tweezers Physics Balancing Forces: Scattering Force- “Radiation Pressure” Gradient Force- Restoring force analogy to spring Figure from: Svoboda and Block, 1994.

Physics, cont. Tight focus = largest gradient force obtained with microscope objective Images from:

Basic Components Objective Lens L2L2 L3L3 L1L1 f 1 + f 2 f3f3 16 cm Image created from Physlet at Physlet by Dr. Wolfgang Christian and Mike Leehttp://webphysics.davidson.edu/Course_Material/Py230L/optics/lenses.htm Laser

NIST Alignment Gained experience with alignment by working on a two-laser trap setup.

Results Single Trap Both Traps Together

NIST Optical Tweezers Applications Liposomes Stretching/Nanotubes Microreaction Vesicles Single Mitochondria Isolation and Sorting Available for PCR and genetic analysis Antigen/Antibody Binding Quantum Dots in Polymersomes Fluorescence Resonance Energy Transfer and single molecule imaging

Davidson Setup

Results Trapping microspheres in 3D 10 mW minimum power from laser (about 2 mW at the slide) Trapping single celled chlamydomonas Image from: rophyceae.html

Future Plans Force calibration Measuring the swimming force of chlamydomonas Flagellar mutants Further in the future: Physics Applications Stretching- elasticity, determining shear moduli Biology Applications More chlamy- phototaxis Virus adhesion

Acknowledgments Dr. Kris Helmerson, Jeff Wells, Lara Crigger, Rani Kishore, Joe Reiner at NIST Dr. John Yukich and Dr. Karen Bernd