Na+ Negatively charged groups (red) Na+ Positively charged counterions Rinse in buffer, add ionic compounds. Positive charges (triangles) stick.

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Na+ Negatively charged groups (red) Na+ Positively charged counterions Rinse in buffer, add ionic compounds. Positive charges (triangles) stick. Negatives (squares) and neutrals (pentagons) do not stick Na+ Sodium ions replaced by positively charged ions Rinse Cation Exchange Chromatography

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